1f3s
From Proteopedia
(New page: 200px<br /><applet load="1f3s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f3s" /> '''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG...) |
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| - | [[Image:1f3s.gif|left|200px]]<br /><applet load="1f3s" size=" | + | [[Image:1f3s.gif|left|200px]]<br /><applet load="1f3s" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1f3s" /> | caption="1f3s" /> | ||
'''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.'''<br /> | '''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to | + | We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit. |
==About this Structure== | ==About this Structure== | ||
| - | 1F3S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1F3S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kettani, A.]] | [[Category: Kettani, A.]] | ||
[[Category: Majumdar, A.]] | [[Category: Majumdar, A.]] | ||
| - | [[Category: Patel, D | + | [[Category: Patel, D J.]] |
[[Category: Skripkin, E.]] | [[Category: Skripkin, E.]] | ||
[[Category: 15n-labeled dna]] | [[Category: 15n-labeled dna]] | ||
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[[Category: uniform 13c]] | [[Category: uniform 13c]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:34:29 2008'' |
Revision as of 10:34, 21 February 2008
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SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.
Overview
We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit.
About this Structure
1F3S is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A two-stranded template-based approach to G.(C-A) triad formation: designing novel structural elements into an existing DNA framework., Kettani A, Basu G, Gorin A, Majumdar A, Skripkin E, Patel DJ, J Mol Biol. 2000 Aug 4;301(1):129-46. PMID:10926497
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