1f90
From Proteopedia
(New page: 200px<br /> <applet load="1f90" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f90, resolution 2.6Å" /> '''FAB FRAGMENT OF MONO...) |
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| - | [[Image:1f90.gif|left|200px]]<br /> | + | [[Image:1f90.gif|left|200px]]<br /><applet load="1f90" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1f90" size=" | + | |
caption="1f90, resolution 2.6Å" /> | caption="1f90, resolution 2.6Å" /> | ||
'''FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE'''<br /> | '''FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of the Fab fragment of a monoclonal | + | The three-dimensional structure of the Fab fragment of a monoclonal antibody (LNKB-2) to human interleukin-2 (IL-2) complexed with a synthetic antigenic nonapeptide, Ac-Lys-Pro-Leu-Glu-Glu-Val-Leu-Asn-Leu-OMe, has been determined at 3.0 A resolution. In the structure, four out of the six hypervariable loops of the Fab (complementarity determining regions [CDRs] L1, H1, H2, and H3) are involved in peptide association through hydrogen bonding, salt bridge formation, and hydrophobic interactions. The Tyr residues in the Fab antigen binding site play a major role in antigen-antibody recognition. The structures of the complexed and uncomplexed Fab were compared. In the antigen binding site the CDR-L1 loop of the antibody shows the largest structural changes upon peptide binding. The peptide adopts a mostly alpha-helical conformation similar to that in the epitope fragment 64-72 of the IL-2 antigen. The side chains of residues Leu 66, Val 69, and Leu 70, which are shielded internally in the IL-2 structure, are involved in interactions with the Fab in the complex studied. This indicates that antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the alpha-helical character of the epitope fragment. |
==About this Structure== | ==About this Structure== | ||
| - | 1F90 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1F90 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F90 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Afonin, P | + | [[Category: Afonin, P V.]] |
| - | [[Category: Duax, W | + | [[Category: Duax, W L.]] |
| - | [[Category: Fokin, A | + | [[Category: Fokin, A V.]] |
| - | [[Category: Ivanov, V | + | [[Category: Ivanov, V T.]] |
[[Category: Li, N.]] | [[Category: Li, N.]] | ||
| - | [[Category: Mareeva, T | + | [[Category: Mareeva, T Y.]] |
| - | [[Category: Mikhailova, I | + | [[Category: Mikhailova, I Y.]] |
| - | [[Category: Mikhaleva, I | + | [[Category: Mikhaleva, I I.]] |
| - | [[Category: Nesmeyanov, V | + | [[Category: Nesmeyanov, V A.]] |
| - | [[Category: Onoprienko, L | + | [[Category: Onoprienko, L V.]] |
| - | [[Category: Pletnev, V | + | [[Category: Pletnev, V Z.]] |
| - | [[Category: Tsigannik, I | + | [[Category: Tsigannik, I N.]] |
[[Category: antigen-binding fragment]] | [[Category: antigen-binding fragment]] | ||
[[Category: antigenic peptide]] | [[Category: antigenic peptide]] | ||
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[[Category: monoclonal antibody]] | [[Category: monoclonal antibody]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:36:14 2008'' |
Revision as of 10:36, 21 February 2008
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FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE
Overview
The three-dimensional structure of the Fab fragment of a monoclonal antibody (LNKB-2) to human interleukin-2 (IL-2) complexed with a synthetic antigenic nonapeptide, Ac-Lys-Pro-Leu-Glu-Glu-Val-Leu-Asn-Leu-OMe, has been determined at 3.0 A resolution. In the structure, four out of the six hypervariable loops of the Fab (complementarity determining regions [CDRs] L1, H1, H2, and H3) are involved in peptide association through hydrogen bonding, salt bridge formation, and hydrophobic interactions. The Tyr residues in the Fab antigen binding site play a major role in antigen-antibody recognition. The structures of the complexed and uncomplexed Fab were compared. In the antigen binding site the CDR-L1 loop of the antibody shows the largest structural changes upon peptide binding. The peptide adopts a mostly alpha-helical conformation similar to that in the epitope fragment 64-72 of the IL-2 antigen. The side chains of residues Leu 66, Val 69, and Leu 70, which are shielded internally in the IL-2 structure, are involved in interactions with the Fab in the complex studied. This indicates that antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the alpha-helical character of the epitope fragment.
About this Structure
1F90 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of an anti-interleukin-2 monoclonal antibody Fab complexed with an antigenic nonapeptide., Afonin PV, Fokin AV, Tsygannik IN, Mikhailova IY, Onoprienko LV, Mikhaleva II, Ivanov VT, Mareeva TY, Nesmeyanov VA, Li N, Pangborn WA, Duax WL, Pletnev VZ, Protein Sci. 2001 Aug;10(8):1514-21. PMID:11468348
Page seeded by OCA on Thu Feb 21 12:36:14 2008
Categories: Mus musculus | Protein complex | Afonin, P V. | Duax, W L. | Fokin, A V. | Ivanov, V T. | Li, N. | Mareeva, T Y. | Mikhailova, I Y. | Mikhaleva, I I. | Nesmeyanov, V A. | Onoprienko, L V. | Pletnev, V Z. | Tsigannik, I N. | Antigen-binding fragment | Antigenic peptide | Interleukin-2 | Monoclonal antibody
