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1o9i
From Proteopedia
(New page: 200px<br /> <applet load="1o9i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1o9i, resolution 1.33Å" /> '''CRYSTAL STRUCTURE O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1O9I is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Lactobacillus_plantarum Lactobacillus plantarum]] with CA, NA, MN3, O and MES as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O9I OCA]]. | + | 1O9I is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Lactobacillus_plantarum Lactobacillus plantarum]] with CA, NA, MN3, O and MES as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Catalase Catalase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6]]. Structure known Active Site: BND. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O9I OCA]]. |
==Reference== | ==Reference== | ||
Outer sphere mutagenesis of Lactobacillus plantarum manganese catalase disrupts the cluster core. Mechanistic implications., Whittaker MM, Barynin VV, Igarashi T, Whittaker JW, Eur J Biochem. 2003 Mar;270(6):1102-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12631270 12631270] | Outer sphere mutagenesis of Lactobacillus plantarum manganese catalase disrupts the cluster core. Mechanistic implications., Whittaker MM, Barynin VV, Igarashi T, Whittaker JW, Eur J Biochem. 2003 Mar;270(6):1102-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12631270 12631270] | ||
| + | [[Category: Catalase]] | ||
[[Category: Lactobacillus plantarum]] | [[Category: Lactobacillus plantarum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: preoxidase]] | [[Category: preoxidase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:01:27 2007'' |
Revision as of 11:56, 30 October 2007
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CRYSTAL STRUCTURE OF THE Y42F MUTANT OF MANGANESE CATALASE FROM LACTOBACILLUS PLANTARUM AT 1.33A RESOLUTION
Overview
X-ray crystallography of the nonheme manganese catalase from Lactobacillus, plantarum (LPC) [Barynin, V.V., Whittaker, M.M., Antonyuk, S.V., Lamzin, V.S., Harrison, P.M., Artymiuk, P.J. & Whittaker, J.W. (2001) Structure9, 725-738] has revealed the structure of the dimanganese redox cluster, together with its protein environment. The oxidized [Mn(III)Mn(III)], cluster is bridged by two solvent molecules (oxo and hydroxo, respectively) together with a micro 1,3 bridging glutamate carboxylate and, is embedded in a web of hydrogen bonds involving an outer sphere tyrosine, residue (Tyr42). A novel homologous expression system has been developed, for production of active recombinant LPC and Tyr42 has been replaced by, phenylalanine using site-directed mutagenesis. Spectroscopic and, ... [(full description)]
About this Structure
1O9I is a [Single protein] structure of sequence from [Lactobacillus plantarum] with CA, NA, MN3, O and MES as [ligands]. Active as [Catalase], with EC number [1.11.1.6]. Structure known Active Site: BND. Full crystallographic information is available from [OCA].
Reference
Outer sphere mutagenesis of Lactobacillus plantarum manganese catalase disrupts the cluster core. Mechanistic implications., Whittaker MM, Barynin VV, Igarashi T, Whittaker JW, Eur J Biochem. 2003 Mar;270(6):1102-16. PMID:12631270
Page seeded by OCA on Tue Oct 30 14:01:27 2007
Categories: Catalase | Lactobacillus plantarum | Single protein | Barynin, V.V. | Whittaker, J.W. | Whittaker, M.M. | CA | MES | MN3 | NA | O | Dimanganese catalase | Hexamer | Metalloenzyme | Oxidoreductase | Preoxidase
