1fjl
From Proteopedia
(New page: 200px<br /><applet load="1fjl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fjl, resolution 2.0Å" /> '''HOMEODOMAIN FROM THE ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1fjl.gif|left|200px]]<br /><applet load="1fjl" size=" | + | [[Image:1fjl.gif|left|200px]]<br /><applet load="1fjl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fjl, resolution 2.0Å" /> | caption="1fjl, resolution 2.0Å" /> | ||
'''HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE'''<br /> | '''HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the paired homeodomain bound to DNA as a | + | The crystal structure of the paired homeodomain bound to DNA as a cooperative dimer has been determined to 2.0 A resolution. Direct contacts between each homeodomain and the DNA are similar to those described previously. In addition, an extensive network of water molecules mediates contacts between the recognition helix and the DNA major groove. Several symmetrical contacts between the two homeodomains underlie the cooperative interaction, and deformations in the DNA structure are necessary for the establishment of these contacts. Comparison with structures of homeodomains bound monomerically to DNA suggests that the binding of a single paired homeodomain can introduce these DNA distortions, thus preparing a template for the cooperative interaction with a second homeodomain. This study shows how the paired (Pax) class homeodomains have achieved cooperativity in DNA binding without the assistance of other domains, thereby enabling the recognition of target sequences that are long enough to ensure specificity. |
==About this Structure== | ==About this Structure== | ||
| - | 1FJL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1FJL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJL OCA]. |
==Reference== | ==Reference== | ||
| Line 16: | Line 16: | ||
[[Category: Guenther, B.]] | [[Category: Guenther, B.]] | ||
[[Category: Kuriyan, J.]] | [[Category: Kuriyan, J.]] | ||
| - | [[Category: Wilson, D | + | [[Category: Wilson, D S.]] |
[[Category: dna]] | [[Category: dna]] | ||
[[Category: dna-binding protein]] | [[Category: dna-binding protein]] | ||
| Line 22: | Line 22: | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:24 2008'' |
Revision as of 10:39, 21 February 2008
|
HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE
Overview
The crystal structure of the paired homeodomain bound to DNA as a cooperative dimer has been determined to 2.0 A resolution. Direct contacts between each homeodomain and the DNA are similar to those described previously. In addition, an extensive network of water molecules mediates contacts between the recognition helix and the DNA major groove. Several symmetrical contacts between the two homeodomains underlie the cooperative interaction, and deformations in the DNA structure are necessary for the establishment of these contacts. Comparison with structures of homeodomains bound monomerically to DNA suggests that the binding of a single paired homeodomain can introduce these DNA distortions, thus preparing a template for the cooperative interaction with a second homeodomain. This study shows how the paired (Pax) class homeodomains have achieved cooperativity in DNA binding without the assistance of other domains, thereby enabling the recognition of target sequences that are long enough to ensure specificity.
About this Structure
1FJL is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
High resolution crystal structure of a paired (Pax) class cooperative homeodomain dimer on DNA., Wilson DS, Guenther B, Desplan C, Kuriyan J, Cell. 1995 Sep 8;82(5):709-19. PMID:7671301
Page seeded by OCA on Thu Feb 21 12:39:24 2008
