1fxd
From Proteopedia
(New page: 200px<br /><applet load="1fxd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fxd, resolution 1.7Å" /> '''REFINED CRYSTAL STRUC...) |
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| - | [[Image:1fxd.gif|left|200px]]<br /><applet load="1fxd" size=" | + | [[Image:1fxd.gif|left|200px]]<br /><applet load="1fxd" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fxd, resolution 1.7Å" /> | caption="1fxd, resolution 1.7Å" /> | ||
'''REFINED CRYSTAL STRUCTURE OF FERREDOXIN II FROM DESULFOVIBRIO GIGAS AT 1.7 ANGSTROMS'''<br /> | '''REFINED CRYSTAL STRUCTURE OF FERREDOXIN II FROM DESULFOVIBRIO GIGAS AT 1.7 ANGSTROMS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of ferredoxin II from Desulfovibrio gigas has been | + | The crystal structure of ferredoxin II from Desulfovibrio gigas has been determined using phasing from anomalous scattering data at a resolution of 1.7 A and refined to an R-factor of 0.157. The molecule has an overall chain fold similar to that of the other bacterial ferredoxins of known structure. The molecule contains a single 3Fe-4S cluster with geometry indistinguishable from the 4Fe-4S clusters, and a disulfide bond near the site corresponding to the position of the second cluster of two-cluster ferredoxins. The cluster is bound by cysteine residues 8, 14 and 50. The side-chain of cysteine 11 extends away from the cluster, but could rotate to become the fourth cysteine ligand in the four-iron form of the molecule given a local adjustment of the polypeptide chain. This residue is modified, however, by what appears to be a methanethiol group. There are a total of eight NH . . . S bonds to the inorganic and cysteine sulfur atoms of the Fe-S cluster. There is an additional residue found that is not reported for the chemical sequence: according to the electron density a valine residue should be inserted after residue 55. |
==About this Structure== | ==About this Structure== | ||
| - | 1FXD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas] with F3S as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1FXD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas] with <scene name='pdbligand=F3S:'>F3S</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FXD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Desulfovibrio gigas]] | [[Category: Desulfovibrio gigas]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Adman, E | + | [[Category: Adman, E T.]] |
| - | [[Category: Jensen, L | + | [[Category: Jensen, L H.]] |
| - | [[Category: Kissinger, C | + | [[Category: Kissinger, C R.]] |
| - | [[Category: Sieker, L | + | [[Category: Sieker, L C.]] |
[[Category: F3S]] | [[Category: F3S]] | ||
[[Category: electron transfer(iron-sulfur)]] | [[Category: electron transfer(iron-sulfur)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:43:38 2008'' |
Revision as of 10:43, 21 February 2008
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REFINED CRYSTAL STRUCTURE OF FERREDOXIN II FROM DESULFOVIBRIO GIGAS AT 1.7 ANGSTROMS
Overview
The crystal structure of ferredoxin II from Desulfovibrio gigas has been determined using phasing from anomalous scattering data at a resolution of 1.7 A and refined to an R-factor of 0.157. The molecule has an overall chain fold similar to that of the other bacterial ferredoxins of known structure. The molecule contains a single 3Fe-4S cluster with geometry indistinguishable from the 4Fe-4S clusters, and a disulfide bond near the site corresponding to the position of the second cluster of two-cluster ferredoxins. The cluster is bound by cysteine residues 8, 14 and 50. The side-chain of cysteine 11 extends away from the cluster, but could rotate to become the fourth cysteine ligand in the four-iron form of the molecule given a local adjustment of the polypeptide chain. This residue is modified, however, by what appears to be a methanethiol group. There are a total of eight NH . . . S bonds to the inorganic and cysteine sulfur atoms of the Fe-S cluster. There is an additional residue found that is not reported for the chemical sequence: according to the electron density a valine residue should be inserted after residue 55.
About this Structure
1FXD is a Single protein structure of sequence from Desulfovibrio gigas with as ligand. Full crystallographic information is available from OCA.
Reference
Refined crystal structure of ferredoxin II from Desulfovibrio gigas at 1.7 A., Kissinger CR, Sieker LC, Adman ET, Jensen LH, J Mol Biol. 1991 Jun 20;219(4):693-715. PMID:2056535
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