1g5h
From Proteopedia
(New page: 200px<br /><applet load="1g5h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g5h, resolution 1.95Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1g5h.gif|left|200px]]<br /><applet load="1g5h" size=" | + | [[Image:1g5h.gif|left|200px]]<br /><applet load="1g5h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1g5h, resolution 1.95Å" /> | caption="1g5h, resolution 1.95Å" /> | ||
'''CRYSTAL STRUCTURE OF THE ACCESSORY SUBUNIT OF MURINE MITOCHONDRIAL POLYMERASE GAMMA'''<br /> | '''CRYSTAL STRUCTURE OF THE ACCESSORY SUBUNIT OF MURINE MITOCHONDRIAL POLYMERASE GAMMA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Polymerase gamma, which replicates and repairs mitochondrial DNA, requires | + | Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through an unusual intermolecular four-helix bundle. A human Pol gamma B mutant lacking the four-helix bundle failed to dimerize in solution or to stimulate the catalytic subunit Pol gamma A, but retained the ability to bind with Pol gamma A to a primer-template construct, indicating that the functional holoenzyme contains two Pol gamma B molecules. Other mutants retained stimulatory activity but lost the ability to bind folded ssDNA. These results suggest that the Pol gamma B dimer contains distinct sites for Pol gamma A binding, dimerization, and DNA binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1G5H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NA and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1G5H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bogenhagen, D | + | [[Category: Bogenhagen, D F.]] |
| - | [[Category: Carrodeguas, J | + | [[Category: Carrodeguas, J A.]] |
[[Category: Kisker, C.]] | [[Category: Kisker, C.]] | ||
[[Category: Theis, K.]] | [[Category: Theis, K.]] | ||
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[[Category: intermolecular four helix bundle]] | [[Category: intermolecular four helix bundle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:46:18 2008'' |
Revision as of 10:46, 21 February 2008
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CRYSTAL STRUCTURE OF THE ACCESSORY SUBUNIT OF MURINE MITOCHONDRIAL POLYMERASE GAMMA
Overview
Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through an unusual intermolecular four-helix bundle. A human Pol gamma B mutant lacking the four-helix bundle failed to dimerize in solution or to stimulate the catalytic subunit Pol gamma A, but retained the ability to bind with Pol gamma A to a primer-template construct, indicating that the functional holoenzyme contains two Pol gamma B molecules. Other mutants retained stimulatory activity but lost the ability to bind folded ssDNA. These results suggest that the Pol gamma B dimer contains distinct sites for Pol gamma A binding, dimerization, and DNA binding.
About this Structure
1G5H is a Single protein structure of sequence from Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure and deletion analysis show that the accessory subunit of mammalian DNA polymerase gamma, Pol gamma B, functions as a homodimer., Carrodeguas JA, Theis K, Bogenhagen DF, Kisker C, Mol Cell. 2001 Jan;7(1):43-54. PMID:11172710
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