This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1g7r

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1g7r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g7r, resolution 2.2&Aring;" /> '''X-RAY STRUCTURE OF TR...)
Line 1: Line 1:
-
[[Image:1g7r.jpg|left|200px]]<br /><applet load="1g7r" size="450" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1g7r.jpg|left|200px]]<br /><applet load="1g7r" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1g7r, resolution 2.2&Aring;" />
caption="1g7r, resolution 2.2&Aring;" />
'''X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B'''<br />
'''X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B'''<br />
==Overview==
==Overview==
-
X-ray structures of the universal translation initiation factor IF2/eIF5B, have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that, facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes, in all three kingdoms of life. The conserved core of IF2/eIF5B consists of, an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed, by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to, a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a, molecular lever, which amplifies a modest conformational change in the, Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a, distance of 90 A from the G domain active center to domain IV. Mechanisms, of GTPase function and ribosome binding are discussed.
+
X-ray structures of the universal translation initiation factor IF2/eIF5B have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes in all three kingdoms of life. The conserved core of IF2/eIF5B consists of an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a molecular lever, which amplifies a modest conformational change in the Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a distance of 90 A from the G domain active center to domain IV. Mechanisms of GTPase function and ribosome binding are discussed.
==About this Structure==
==About this Structure==
-
1G7R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G7R OCA].
+
1G7R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7R OCA].
==Reference==
==Reference==
Line 13: Line 13:
[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Single protein]]
[[Category: Single protein]]
-
[[Category: Burley, S.K.]]
+
[[Category: Burley, S K.]]
[[Category: Cao, C.]]
[[Category: Cao, C.]]
-
[[Category: Dever, T.E.]]
+
[[Category: Dever, T E.]]
[[Category: Roll-Mecak, A.]]
[[Category: Roll-Mecak, A.]]
[[Category: translational gtpase]]
[[Category: translational gtpase]]
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:47:30 2007''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:47:02 2008''

Revision as of 10:47, 21 February 2008

Image:1g7r.jpg

1g7r, resolution 2.2Å

Drag the structure with the mouse to rotate

X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B

Overview

X-ray structures of the universal translation initiation factor IF2/eIF5B have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes in all three kingdoms of life. The conserved core of IF2/eIF5B consists of an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a molecular lever, which amplifies a modest conformational change in the Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a distance of 90 A from the G domain active center to domain IV. Mechanisms of GTPase function and ribosome binding are discussed.

About this Structure

1G7R is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.

Reference

X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding., Roll-Mecak A, Cao C, Dever TE, Burley SK, Cell. 2000 Nov 22;103(5):781-92. PMID:11114334

Page seeded by OCA on Thu Feb 21 12:47:02 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g7r"
Personal tools