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1bk0
From Proteopedia
(New page: 200px<br /> <applet load="1bk0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bk0, resolution 1.3Å" /> '''ISOPENICILLIN N SYNT...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1BK0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with SO4, FE and ACV as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BK0 OCA]]. | + | 1BK0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with SO4, FE and ACV as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: SA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BK0 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: penicillin biosynthesis]] | [[Category: penicillin biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:07:38 2007'' |
Revision as of 12:02, 30 October 2007
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ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (ACV-FE COMPLEX)
Overview
The biosynthesis of penicillin and cephalosporin antibiotics in, microorganisms requires the formation of the bicyclic nucleus of, penicillin. Isopenicillin N synthase (IPNS), a non-haem iron-dependent, oxidase, catalyses the reaction of a tripeptide, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV), and dioxygen to, form isopenicillin N and two water molecules. Mechanistic studies suggest, the reaction is initiated by ligation of the substrate thiolate to the, iron centre, and proceeds through an enzyme-bound monocyclic intermediate., Here we report the crystal structure of IPNS complexed to ferrous iron and, ACV, determined to 1.3 A resolution. Based on the structure, we propose a, mechanism for penicillin formation that involves ligation of ACV to the, iron centre, creating a ... [(full description)]
About this Structure
1BK0 is a [Single protein] structure of sequence from [Emericella nidulans] with SO4, FE and ACV as [ligands]. Structure known Active Site: SA. Full crystallographic information is available from [OCA].
Reference
Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation., Roach PL, Clifton IJ, Hensgens CM, Shibata N, Schofield CJ, Hajdu J, Baldwin JE, Nature. 1997 Jun 19;387(6635):827-30. PMID:9194566
Page seeded by OCA on Tue Oct 30 14:07:38 2007
