1oh0
From Proteopedia
(New page: 200px<br /> <applet load="1oh0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oh0, resolution 1.1Å" /> '''CRYSTAL STRUCTURE OF...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1OH0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with EQU and BME as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 4TSU. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OH0 OCA]]. | + | 1OH0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with EQU and BME as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 4TSU. Active as [[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OH0 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Steroid Delta-isomerase]] | ||
[[Category: Byun, M.]] | [[Category: Byun, M.]] | ||
[[Category: Cha, S.S.]] | [[Category: Cha, S.S.]] | ||
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[[Category: pi]] | [[Category: pi]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:08:01 2007'' |
Revision as of 12:03, 30 October 2007
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CRYSTAL STRUCTURE OF KETOSTEROID ISOMERASE COMPLEXED WITH EQUILENIN
Overview
Bacterial Delta5-3-ketosteroid isomerase (KSI) catalyzes a stereospecific, isomerization of steroid substrates at an extremely fast rate, overcoming, a large disparity of pKa values between a catalytic residue and its, target. The crystal structures of KSI from Pseudomonas putida and of the, enzyme in complex with equilenin, an analogue of the reaction, intermediate, have been determined at 1.9 and 2.5 A resolution, respectively. The structures reveal that the side chains of Tyr14 and, Asp99 (a newly identified catalytic residue) form hydrogen bonds directly, with the oxyanion of the bound inhibitor in a completely apolar milieu of, the active site. No water molecule is found at the active site, and the, access of bulk solvent is blocked by a layer of apolar residues. Asp99 is, surrounded ... [(full description)]
About this Structure
1OH0 is a [Single protein] structure of sequence from [Pseudomonas putida] with EQU and BME as [ligands]. This structure superseeds the now removed PDB entry 4TSU. Active as [Steroid Delta-isomerase], with EC number [5.3.3.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
High-resolution crystal structures of delta5-3-ketosteroid isomerase with and without a reaction intermediate analogue., Kim SW, Cha SS, Cho HS, Kim JS, Ha NC, Cho MJ, Joo S, Kim KK, Choi KY, Oh BH, Biochemistry. 1997 Nov 18;36(46):14030-6. PMID:9369474
Page seeded by OCA on Tue Oct 30 14:08:01 2007
Categories: Pseudomonas putida | Single protein | Steroid Delta-isomerase | Byun, M. | Cha, S.S. | Kim, K.H. | Oh, B.H. | BME | EQU | Equilenin | Isomerase | Ketosteroid isomerase | Ksi | Lbhb | Pi
