1gg3
From Proteopedia
(New page: 200px<br /> <applet load="1gg3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gg3, resolution 2.80Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1gg3.gif|left|200px]]<br /> | + | [[Image:1gg3.gif|left|200px]]<br /><applet load="1gg3" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1gg3" size=" | + | |
caption="1gg3, resolution 2.80Å" /> | caption="1gg3, resolution 2.80Å" /> | ||
'''CRYSTAL STRUCTURE OF THE PROTEIN 4.1R MEMBRANE BINDING DOMAIN'''<br /> | '''CRYSTAL STRUCTURE OF THE PROTEIN 4.1R MEMBRANE BINDING DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the core domain (N-terminal 30 kDa domain) of | + | The crystal structure of the core domain (N-terminal 30 kDa domain) of cytoskeletal protein 4.1R has been determined and shows a cloverleaf-like architecture. Each lobe of the cloverleaf contains a specific binding site for either band 3, glycophorin C/D or p55. At a central region of the molecule near where the three lobes are joined are two separate calmodulin (CaM) binding regions. One of these is composed primarily of an alpha-helix and is Ca 2+ insensitive; the other takes the form of an extended structure and its binding with CaM is dramatically enhanced by the presence of Ca 2+, resulting in the weakening of protein 4.1R binding to its target proteins. This novel architecture, in which the three lobes bind with three membrane associated proteins, and the location of calmodulin binding sites provide insight into how the protein 4.1R core domain interacts with membrane proteins and dynamically regulates cell shape in response to changes in intracellular Ca2+ levels. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1GG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GG3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Han, B | + | [[Category: Han, B G.]] |
[[Category: 30kd]] | [[Category: 30kd]] | ||
| - | [[Category: 4 | + | [[Category: 4 1r]] |
[[Category: blood]] | [[Category: blood]] | ||
[[Category: calmodulin]] | [[Category: calmodulin]] | ||
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[[Category: n-terminal domain]] | [[Category: n-terminal domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:49:48 2008'' |
Revision as of 10:49, 21 February 2008
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CRYSTAL STRUCTURE OF THE PROTEIN 4.1R MEMBRANE BINDING DOMAIN
Contents |
Overview
The crystal structure of the core domain (N-terminal 30 kDa domain) of cytoskeletal protein 4.1R has been determined and shows a cloverleaf-like architecture. Each lobe of the cloverleaf contains a specific binding site for either band 3, glycophorin C/D or p55. At a central region of the molecule near where the three lobes are joined are two separate calmodulin (CaM) binding regions. One of these is composed primarily of an alpha-helix and is Ca 2+ insensitive; the other takes the form of an extended structure and its binding with CaM is dramatically enhanced by the presence of Ca 2+, resulting in the weakening of protein 4.1R binding to its target proteins. This novel architecture, in which the three lobes bind with three membrane associated proteins, and the location of calmodulin binding sites provide insight into how the protein 4.1R core domain interacts with membrane proteins and dynamically regulates cell shape in response to changes in intracellular Ca2+ levels.
Disease
Known disease associated with this structure: Elliptocytosis-1 OMIM:[130500]
About this Structure
1GG3 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Protein 4.1R core domain structure and insights into regulation of cytoskeletal organization., Han BG, Nunomura W, Takakuwa Y, Mohandas N, Jap BK, Nat Struct Biol. 2000 Oct;7(10):871-5. PMID:11017195
Page seeded by OCA on Thu Feb 21 12:49:48 2008
Categories: Homo sapiens | Single protein | Han, B G. | 30kd | 4 1r | Blood | Calmodulin | Membrane | N-terminal domain
