1afw
From Proteopedia
(New page: 200px<br /> <applet load="1afw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1afw, resolution 1.8Å" /> '''THE 1.8 ANGSTROM CRY...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1AFW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with MRD as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.16 2.3.1.16]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AFW OCA]]. | + | 1AFW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with MRD as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Acetyl-CoA_C-acyltransferase Acetyl-CoA C-acyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.16 2.3.1.16]]. Structure known Active Sites: AVA and AVB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AFW OCA]]. |
==Reference== | ==Reference== | ||
The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism., Mathieu M, Modis Y, Zeelen JP, Engel CK, Abagyan RA, Ahlberg A, Rasmussen B, Lamzin VS, Kunau WH, Wierenga RK, J Mol Biol. 1997 Oct 31;273(3):714-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9402066 9402066] | The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism., Mathieu M, Modis Y, Zeelen JP, Engel CK, Abagyan RA, Ahlberg A, Rasmussen B, Lamzin VS, Kunau WH, Wierenga RK, J Mol Biol. 1997 Oct 31;273(3):714-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9402066 9402066] | ||
| + | [[Category: Acetyl-CoA C-acyltransferase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 21: | ||
[[Category: thiolase]] | [[Category: thiolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:11:54 2007'' |
Revision as of 12:07, 30 October 2007
|
THE 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE DIMERIC PEROXISOMAL THIOLASE OF SACCHAROMYCES CEREVISIAE
Overview
The dimeric, peroxisomal 3-ketoacyl-CoA thiolase catalyses the conversion, of 3-ketoacyl-CoA into acyl-CoA, which is shorter by two carbon atoms., This reaction is the last step of the beta-oxidation pathway. The crystal, structure of unliganded peroxisomal thiolase of the yeast Saccharomyces, cerevisiae has been refined at 1.8 A resolution. An unusual feature of, this structure is the presence of two helices, completely buried in the, dimer and sandwiched between two beta-sheets. The analysis of the, structure shows that the sequences of these helices are not hydrophobic, but generate two amphipathic helices. The helix in the N-terminal domain, exposes the polar side-chains to a cavity at the dimer interface, filled, with structured water molecules. The central helix in the C-terminal, ... [(full description)]
About this Structure
1AFW is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with MRD as [ligand]. Active as [Acetyl-CoA C-acyltransferase], with EC number [2.3.1.16]. Structure known Active Sites: AVA and AVB. Full crystallographic information is available from [OCA].
Reference
The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism., Mathieu M, Modis Y, Zeelen JP, Engel CK, Abagyan RA, Ahlberg A, Rasmussen B, Lamzin VS, Kunau WH, Wierenga RK, J Mol Biol. 1997 Oct 31;273(3):714-28. PMID:9402066
Page seeded by OCA on Tue Oct 30 14:11:54 2007
