1h3h
From Proteopedia
(New page: 200px<br /><applet load="1h3h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h3h" /> '''STRUCTURAL BASIS FOR SPECIFIC RECOGNITION OF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1h3h.jpg|left|200px]]<br /><applet load="1h3h" size=" | + | [[Image:1h3h.jpg|left|200px]]<br /><applet load="1h3h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h3h" /> | caption="1h3h" /> | ||
'''STRUCTURAL BASIS FOR SPECIFIC RECOGNITION OF AN RXXK-CONTAINING SLP-76 PEPTIDE BY THE GADS C-TERMINAL SH3 DOMAIN'''<br /> | '''STRUCTURAL BASIS FOR SPECIFIC RECOGNITION OF AN RXXK-CONTAINING SLP-76 PEPTIDE BY THE GADS C-TERMINAL SH3 DOMAIN'''<br /> | ||
| + | |||
| + | ==Overview== | ||
| + | The SH3 domain, which normally recognizes proline-rich sequences, has the potential to bind motifs with an RxxK consensus. To explore this novel specificity, we have determined the solution structure of the Gads T cell adaptor C-terminal SH3 domain in complex with an RSTK-containing peptide, representing its physiological binding site on the SLP-76 docking protein. The SLP-76 peptide engages four distinct binding pockets on the surface of the Gads SH3 domain and upon binding adopts a unique structure characterized by a right-handed 3(10) helix at the RSTK locus, in contrast to the left-handed polyproline type II helix formed by canonical proline-rich SH3 ligands. The structure, and supporting mutagenesis and peptide binding data, reveal a novel mode of ligand recognition by SH3 domains. | ||
==About this Structure== | ==About this Structure== | ||
| - | 1H3H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1H3H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H3H OCA]. |
| + | |||
| + | ==Reference== | ||
| + | Structural basis for specific binding of the Gads SH3 domain to an RxxK motif-containing SLP-76 peptide: a novel mode of peptide recognition., Liu Q, Berry D, Nash P, Pawson T, McGlade CJ, Li SS, Mol Cell. 2003 Feb;11(2):471-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12620234 12620234] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Berry, D.]] | [[Category: Berry, D.]] | ||
| - | [[Category: Li, S | + | [[Category: Li, S S.]] |
[[Category: Liu, Q.]] | [[Category: Liu, Q.]] | ||
| - | [[Category: Mcglade, C | + | [[Category: Mcglade, C J.]] |
[[Category: Nash, P.]] | [[Category: Nash, P.]] | ||
[[Category: Pawson, T.]] | [[Category: Pawson, T.]] | ||
| Line 18: | Line 24: | ||
[[Category: t-cell signaling]] | [[Category: t-cell signaling]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:57:00 2008'' |
Revision as of 10:57, 21 February 2008
|
STRUCTURAL BASIS FOR SPECIFIC RECOGNITION OF AN RXXK-CONTAINING SLP-76 PEPTIDE BY THE GADS C-TERMINAL SH3 DOMAIN
Overview
The SH3 domain, which normally recognizes proline-rich sequences, has the potential to bind motifs with an RxxK consensus. To explore this novel specificity, we have determined the solution structure of the Gads T cell adaptor C-terminal SH3 domain in complex with an RSTK-containing peptide, representing its physiological binding site on the SLP-76 docking protein. The SLP-76 peptide engages four distinct binding pockets on the surface of the Gads SH3 domain and upon binding adopts a unique structure characterized by a right-handed 3(10) helix at the RSTK locus, in contrast to the left-handed polyproline type II helix formed by canonical proline-rich SH3 ligands. The structure, and supporting mutagenesis and peptide binding data, reveal a novel mode of ligand recognition by SH3 domains.
About this Structure
1H3H is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for specific binding of the Gads SH3 domain to an RxxK motif-containing SLP-76 peptide: a novel mode of peptide recognition., Liu Q, Berry D, Nash P, Pawson T, McGlade CJ, Li SS, Mol Cell. 2003 Feb;11(2):471-81. PMID:12620234
Page seeded by OCA on Thu Feb 21 12:57:00 2008
Categories: Homo sapiens | Mus musculus | Protein complex | Berry, D. | Li, S S. | Liu, Q. | Mcglade, C J. | Nash, P. | Pawson, T. | Sh2 domain | Sh3 domain | T-cell signaling
