1h8p
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Bovine seminal plasma PDC-109 binds to sperm surface choline lipids and | + | Bovine seminal plasma PDC-109 binds to sperm surface choline lipids and promotes sperm capacitation by stimulating the efflux of cholesterol and phospholipids. The structure of PDC-109 with bound phosphorylcholine was solved using MAD data of a single platinum site. Its two globular (40 x 50 x 20 A(3)) Fn2 domains are linked and clustered by a short polypeptide. The choline binding sites lie at the same face of the molecule. Phosphorylcholine binds to the Fn2 domains through a cation-pi interaction between the quaternary ammonium group and a core tryptophan, plus hydrogen bonding between hydroxyls of exposed tyrosines and the phosphate group. The structure of the PDC-109-oPC complex provides a structural ground for the sperm membrane-coating mechanism underlying PDC-109-induced capacitation. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Calvete, J | + | [[Category: Calvete, J J.]] |
[[Category: Fernandez-Tornero, C.]] | [[Category: Fernandez-Tornero, C.]] | ||
[[Category: Romero, A.]] | [[Category: Romero, A.]] | ||
| - | [[Category: Wah, D | + | [[Category: Wah, D A.]] |
[[Category: PC]] | [[Category: PC]] | ||
[[Category: phosphorylcholine-binding protein]] | [[Category: phosphorylcholine-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:58:42 2008'' |
Revision as of 10:58, 21 February 2008
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BULL SEMINAL PLASMA PDC-109 FIBRONECTIN TYPE II MODULE
Overview
Bovine seminal plasma PDC-109 binds to sperm surface choline lipids and promotes sperm capacitation by stimulating the efflux of cholesterol and phospholipids. The structure of PDC-109 with bound phosphorylcholine was solved using MAD data of a single platinum site. Its two globular (40 x 50 x 20 A(3)) Fn2 domains are linked and clustered by a short polypeptide. The choline binding sites lie at the same face of the molecule. Phosphorylcholine binds to the Fn2 domains through a cation-pi interaction between the quaternary ammonium group and a core tryptophan, plus hydrogen bonding between hydroxyls of exposed tyrosines and the phosphate group. The structure of the PDC-109-oPC complex provides a structural ground for the sperm membrane-coating mechanism underlying PDC-109-induced capacitation.
About this Structure
1H8P is a Single protein structure of sequence from Bos taurus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Sperm coating mechanism from the 1.8 A crystal structure of PDC-109-phosphorylcholine complex., Wah DA, Fernandez-Tornero C, Sanz L, Romero A, Calvete JJ, Structure. 2002 Apr;10(4):505-14. PMID:11937055
Page seeded by OCA on Thu Feb 21 12:58:42 2008
