1het
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Atomic (1 A) resolution x-ray structures of horse liver alcohol | + | Atomic (1 A) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Equus caballus]] | [[Category: Equus caballus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Adolph, H | + | [[Category: Adolph, H W.]] |
| - | [[Category: Cedergen-Zeppezauer, E | + | [[Category: Cedergen-Zeppezauer, E S.]] |
| - | [[Category: Lamzin, V | + | [[Category: Lamzin, V S.]] |
[[Category: Meijers, R.]] | [[Category: Meijers, R.]] | ||
[[Category: Merli, A.]] | [[Category: Merli, A.]] | ||
| - | [[Category: Morris, R | + | [[Category: Morris, R J.]] |
[[Category: MRD]] | [[Category: MRD]] | ||
[[Category: NAD]] | [[Category: NAD]] | ||
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[[Category: oxidoreductase(nad(a)-choh(d))]] | [[Category: oxidoreductase(nad(a)-choh(d))]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:00:32 2008'' |
Revision as of 11:00, 21 February 2008
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ATOMIC X-RAY STRUCTURE OF LIVER ALCOHOL DEHYDROGENASE CONTAINING A HYDROXIDE ADDUCT TO NADH
Overview
Atomic (1 A) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.
About this Structure
1HET is a Single protein structure of sequence from Equus caballus with , and as ligands. Active as Alcohol dehydrogenase, with EC number 1.1.1.1 Known structural/functional Sites: , , , , and . Full crystallographic information is available from OCA.
Reference
On the enzymatic activation of NADH., Meijers R, Morris RJ, Adolph HW, Merli A, Lamzin VS, Cedergren-Zeppezauer ES, J Biol Chem. 2001 Mar 23;276(12):9316-21. Epub 2000 Dec 28. PMID:11134046
Page seeded by OCA on Thu Feb 21 13:00:32 2008
