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1hi9

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==Overview==
==Overview==
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Bacillus subtilis DppA is a binuclear zinc-dependent, D-specific, aminopeptidase. The X-ray structure of the enzyme has been determined at, 2.4 A resolution by a three-wavelength MAD experiment. The structure, reveals that DppA is a new example of a 'self-compartmentalizing, protease', a family of proteolytic complexes. Proteasomes are the most, extensively studied representatives of this family. The DppA enzyme is, composed of identical 30 kDa subunits organized in a decamer with 52, point-group symmetry. A 20 A wide channel runs through the complex, giving, access to a central chamber holding the active sites. The structure shows, DppA to be a prototype of a new family of metalloaminopeptidases, characterized by the SXDXEG key sequence.
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Bacillus subtilis DppA is a binuclear zinc-dependent, D-specific aminopeptidase. The X-ray structure of the enzyme has been determined at 2.4 A resolution by a three-wavelength MAD experiment. The structure reveals that DppA is a new example of a 'self-compartmentalizing protease', a family of proteolytic complexes. Proteasomes are the most extensively studied representatives of this family. The DppA enzyme is composed of identical 30 kDa subunits organized in a decamer with 52 point-group symmetry. A 20 A wide channel runs through the complex, giving access to a central chamber holding the active sites. The structure shows DppA to be a prototype of a new family of metalloaminopeptidases characterized by the SXDXEG key sequence.
==About this Structure==
==About this Structure==
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Beeumen, J.Van.]]
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[[Category: Beeumen, J Van.]]
[[Category: Bompard-Gilles, C.]]
[[Category: Bompard-Gilles, C.]]
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[[Category: Frere, J.M.]]
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[[Category: Frere, J M.]]
[[Category: Goffin, C.]]
[[Category: Goffin, C.]]
[[Category: Remaut, H.]]
[[Category: Remaut, H.]]
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[[Category: self-compartmentalizing]]
[[Category: self-compartmentalizing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:49:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:01:29 2008''

Revision as of 11:01, 21 February 2008


1hi9, resolution 2.40Å

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ZN-DEPENDENT D-AMINOPEPTIDASE DPPA FROM BACILLUS SUBTILIS, A SELF-COMPARTMENTALIZING PROTEASE.

Overview

Bacillus subtilis DppA is a binuclear zinc-dependent, D-specific aminopeptidase. The X-ray structure of the enzyme has been determined at 2.4 A resolution by a three-wavelength MAD experiment. The structure reveals that DppA is a new example of a 'self-compartmentalizing protease', a family of proteolytic complexes. Proteasomes are the most extensively studied representatives of this family. The DppA enzyme is composed of identical 30 kDa subunits organized in a decamer with 52 point-group symmetry. A 20 A wide channel runs through the complex, giving access to a central chamber holding the active sites. The structure shows DppA to be a prototype of a new family of metalloaminopeptidases characterized by the SXDXEG key sequence.

About this Structure

1HI9 is a Single protein structure of sequence from Bacillus subtilis with as ligand. Known structural/functional Sites: , , , , , , , , and . Full crystallographic information is available from OCA.

Reference

Structure of the Bacillus subtilis D-aminopeptidase DppA reveals a novel self-compartmentalizing protease., Remaut H, Bompard-Gilles C, Goffin C, Frere JM, Van Beeumen J, Nat Struct Biol. 2001 Aug;8(8):674-8. PMID:11473256

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