1hys
From Proteopedia
(New page: 200px<br /> <applet load="1hys" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hys, resolution 3.0Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1hys.gif|left|200px]]<br /> | + | [[Image:1hys.gif|left|200px]]<br /><applet load="1hys" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1hys" size=" | + | |
caption="1hys, resolution 3.0Å" /> | caption="1hys, resolution 3.0Å" /> | ||
'''CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA'''<br /> | '''CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the 3.0 A resolution structure of wild-type HIV-1 | + | We have determined the 3.0 A resolution structure of wild-type HIV-1 reverse transcriptase in complex with an RNA:DNA oligonucleotide whose sequence includes a purine-rich segment from the HIV-1 genome called the polypurine tract (PPT). The PPT is resistant to ribonuclease H (RNase H) cleavage and is used as a primer for second DNA strand synthesis. The 'RNase H primer grip', consisting of amino acids that interact with the DNA primer strand, may contribute to RNase H catalysis and cleavage specificity. Cleavage specificity is also controlled by the width of the minor groove and the trajectory of the RNA:DNA, both of which are sequence dependent. An unusual 'unzipping' of 7 bp occurs in the adenine stretch of the PPT: an unpaired base on the template strand takes the base pairing out of register and then, following two offset base pairs, an unpaired base on the primer strand re-establishes the normal register. The structural aberration extends to the RNase H active site and may play a role in the resistance of PPT to RNase H cleavage. |
==About this Structure== | ==About this Structure== | ||
| - | 1HYS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Full crystallographic information is available from [http:// | + | 1HYS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HYS OCA]. |
==Reference== | ==Reference== | ||
| Line 17: | Line 16: | ||
[[Category: RNA-directed DNA polymerase]] | [[Category: RNA-directed DNA polymerase]] | ||
[[Category: Arnold, E.]] | [[Category: Arnold, E.]] | ||
| - | [[Category: Boyer, P | + | [[Category: Boyer, P L.]] |
[[Category: Das, K.]] | [[Category: Das, K.]] | ||
[[Category: Ding, J.]] | [[Category: Ding, J.]] | ||
| - | [[Category: Hughes, S | + | [[Category: Hughes, S H.]] |
| - | [[Category: Jr., A | + | [[Category: Jr., A D.Clark.]] |
| - | [[Category: Sarafianos, S | + | [[Category: Sarafianos, S G.]] |
[[Category: Tantillo, C.]] | [[Category: Tantillo, C.]] | ||
[[Category: Whitcomb, J.]] | [[Category: Whitcomb, J.]] | ||
| Line 33: | Line 32: | ||
[[Category: unpaired base]] | [[Category: unpaired base]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:06:17 2008'' |
Revision as of 11:06, 21 February 2008
|
CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA
Overview
We have determined the 3.0 A resolution structure of wild-type HIV-1 reverse transcriptase in complex with an RNA:DNA oligonucleotide whose sequence includes a purine-rich segment from the HIV-1 genome called the polypurine tract (PPT). The PPT is resistant to ribonuclease H (RNase H) cleavage and is used as a primer for second DNA strand synthesis. The 'RNase H primer grip', consisting of amino acids that interact with the DNA primer strand, may contribute to RNase H catalysis and cleavage specificity. Cleavage specificity is also controlled by the width of the minor groove and the trajectory of the RNA:DNA, both of which are sequence dependent. An unusual 'unzipping' of 7 bp occurs in the adenine stretch of the PPT: an unpaired base on the template strand takes the base pairing out of register and then, following two offset base pairs, an unpaired base on the primer strand re-establishes the normal register. The structural aberration extends to the RNase H active site and may play a role in the resistance of PPT to RNase H cleavage.
About this Structure
1HYS is a Protein complex structure of sequences from Human immunodeficiency virus 1 and Mus musculus. Active as RNA-directed DNA polymerase, with EC number 2.7.7.49 Full crystallographic information is available from OCA.
Reference
Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA., Sarafianos SG, Das K, Tantillo C, Clark AD Jr, Ding J, Whitcomb JM, Boyer PL, Hughes SH, Arnold E, EMBO J. 2001 Mar 15;20(6):1449-61. PMID:11250910
Page seeded by OCA on Thu Feb 21 13:06:17 2008
Categories: Human immunodeficiency virus 1 | Mus musculus | Protein complex | RNA-directed DNA polymerase | Arnold, E. | Boyer, P L. | Das, K. | Ding, J. | Hughes, S H. | Jr., A D.Clark. | Sarafianos, S G. | Tantillo, C. | Whitcomb, J. | Polypurine tract | Ppt | Protein-nucleic acid complex | Rna:dna | Rnase h | Rnase h primer grip | Unpaired base
