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1i4o
From Proteopedia
(New page: 200px<br /> <applet load="1i4o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i4o, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1i4o.gif|left|200px]]<br /> | + | [[Image:1i4o.gif|left|200px]]<br /><applet load="1i4o" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1i4o" size=" | + | |
caption="1i4o, resolution 2.4Å" /> | caption="1i4o, resolution 2.4Å" /> | ||
'''CRYSTAL STRUCTURE OF THE XIAP/CASPASE-7 COMPLEX'''<br /> | '''CRYSTAL STRUCTURE OF THE XIAP/CASPASE-7 COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The inhibitor of apoptosis proteins (IAPs) represent the only endogenous | + | The inhibitor of apoptosis proteins (IAPs) represent the only endogenous caspase inhibitors and are characterized by the presence of baculoviral IAP repeats (BIRs). Here, we report the crystal structure of the complex between human caspase-7 and XIAP (BIR2 and the proceeding linker). The structure surprisingly reveals that the linker is the only contacting element for the caspase, while the BIR2 domain is invisible in the crystal. The linker interacts with and blocks the substrate groove of the caspase in a backward fashion, distinct from substrate recognition. Structural analyses suggest that the linker is the energetic and specificity determinant of the interaction. Further biochemical characterizations clearly establish that the linker harbors the major energetic determinant, while the BIR2 domain serves as a regulatory element for caspase binding and Smac neutralization. |
==Disease== | ==Disease== | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1I4O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1I4O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Huang, Y.]] | [[Category: Huang, Y.]] | ||
| - | [[Category: Myszka, D | + | [[Category: Myszka, D G.]] |
| - | [[Category: Park, Y | + | [[Category: Park, Y C.]] |
| - | [[Category: Rich, R | + | [[Category: Rich, R L.]] |
[[Category: Segal, D.]] | [[Category: Segal, D.]] | ||
[[Category: Wu, H.]] | [[Category: Wu, H.]] | ||
[[Category: protease-inhibitor]] | [[Category: protease-inhibitor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:07:57 2008'' |
Revision as of 11:07, 21 February 2008
|
CRYSTAL STRUCTURE OF THE XIAP/CASPASE-7 COMPLEX
Contents |
Overview
The inhibitor of apoptosis proteins (IAPs) represent the only endogenous caspase inhibitors and are characterized by the presence of baculoviral IAP repeats (BIRs). Here, we report the crystal structure of the complex between human caspase-7 and XIAP (BIR2 and the proceeding linker). The structure surprisingly reveals that the linker is the only contacting element for the caspase, while the BIR2 domain is invisible in the crystal. The linker interacts with and blocks the substrate groove of the caspase in a backward fashion, distinct from substrate recognition. Structural analyses suggest that the linker is the energetic and specificity determinant of the interaction. Further biochemical characterizations clearly establish that the linker harbors the major energetic determinant, while the BIR2 domain serves as a regulatory element for caspase binding and Smac neutralization.
Disease
Known diseases associated with this structure: Lymphoproliferative syndrome, X-linked, 2 OMIM:[300079]
About this Structure
1I4O is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of caspase inhibition by XIAP: differential roles of the linker versus the BIR domain., Huang Y, Park YC, Rich RL, Segal D, Myszka DG, Wu H, Cell. 2001 Mar 9;104(5):781-90. PMID:11257231
Page seeded by OCA on Thu Feb 21 13:07:57 2008
