1iai
From Proteopedia
(New page: 200px<br /> <applet load="1iai" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iai, resolution 2.9Å" /> '''IDIOTYPE-ANTI-IDIOTY...) |
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| - | [[Image:1iai.gif|left|200px]]<br /> | + | [[Image:1iai.gif|left|200px]]<br /><applet load="1iai" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1iai" size=" | + | |
caption="1iai, resolution 2.9Å" /> | caption="1iai, resolution 2.9Å" /> | ||
'''IDIOTYPE-ANTI-IDIOTYPE FAB COMPLEX'''<br /> | '''IDIOTYPE-ANTI-IDIOTYPE FAB COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Anti-idiotypic monoclonal antibody 409.5.3 is raised against an antibody | + | Anti-idiotypic monoclonal antibody 409.5.3 is raised against an antibody that neutralizes feline infectious peritonitis virus. This antibody, used as an immunogen, elicits the production of anti-anti-idiotypic antibodies that in turn neutralize the virus. The crystal structure of the complex between anti-idiotypic Fab 409.5.3 and idiotypic Fab fragment of virus-neutralizing antibody has been solved by molecular replacement using real-space Patterson search and filtering by Patterson correlation-coefficient refinement. The structure has been refined to an R value of 0.21 based on 21,310 unique reflections between 40.0 and 2.9 A. The three-dimensional structure reveals extensive, specific interactions that involve 118 van der Waals contacts and at least 9 probable hydrogen bonds. The two Fabs are rotated 61 degrees with respect to each other around the approximate long axis of the complex and are within 26 degrees being aligned along their major axes. |
==About this Structure== | ==About this Structure== | ||
| - | 1IAI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1IAI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: complex (immunoglobulin igg1/igg2a)]] | [[Category: complex (immunoglobulin igg1/igg2a)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:49 2008'' |
Revision as of 11:09, 21 February 2008
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IDIOTYPE-ANTI-IDIOTYPE FAB COMPLEX
Overview
Anti-idiotypic monoclonal antibody 409.5.3 is raised against an antibody that neutralizes feline infectious peritonitis virus. This antibody, used as an immunogen, elicits the production of anti-anti-idiotypic antibodies that in turn neutralize the virus. The crystal structure of the complex between anti-idiotypic Fab 409.5.3 and idiotypic Fab fragment of virus-neutralizing antibody has been solved by molecular replacement using real-space Patterson search and filtering by Patterson correlation-coefficient refinement. The structure has been refined to an R value of 0.21 based on 21,310 unique reflections between 40.0 and 2.9 A. The three-dimensional structure reveals extensive, specific interactions that involve 118 van der Waals contacts and at least 9 probable hydrogen bonds. The two Fabs are rotated 61 degrees with respect to each other around the approximate long axis of the complex and are within 26 degrees being aligned along their major axes.
About this Structure
1IAI is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of an idiotype-anti-idiotype Fab complex., Ban N, Escobar C, Garcia R, Hasel K, Day J, Greenwood A, McPherson A, Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1604-8. PMID:8127852
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