1if1
From Proteopedia
(New page: 200px<br /><applet load="1if1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1if1, resolution 3.000Å" /> '''INTERFERON REGULATO...) |
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| - | [[Image:1if1.gif|left|200px]]<br /><applet load="1if1" size=" | + | [[Image:1if1.gif|left|200px]]<br /><applet load="1if1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1if1, resolution 3.000Å" /> | caption="1if1, resolution 3.000Å" /> | ||
'''INTERFERON REGULATORY FACTOR 1 (IRF-1) COMPLEX WITH DNA'''<br /> | '''INTERFERON REGULATORY FACTOR 1 (IRF-1) COMPLEX WITH DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The family of interferon regulatory factor (IRF) transcription factors is | + | The family of interferon regulatory factor (IRF) transcription factors is important in the regulation of interferons in response to infection by virus and in the regulation of interferon-inducible genes. The IRF family is characterized by a unique 'tryptophan cluster' DNA-binding region. Here we report the crystal structure of the IRF-1 region bound to the natural positive regulatory domain I (PRD I) DNA element from the interferon-beta promoter. The structure provides the first three-dimensional view of a member of the growing IRF family, revealing a new helix-turn-helix motif that latches onto DNA through three of the five conserved tryptophans. The motif selects a short GAAA core sequence through an obliquely angled recognition helix, with an accompanying bending of the DNA axis in the direction of the protein. Together, these features suggest a basis for the occurrence of GAAA repeats within IRF response elements and provide clues to the assembly of the higher-order interferon-beta enhancesome. |
==About this Structure== | ==About this Structure== | ||
| - | 1IF1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1IF1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Escalante, A | + | [[Category: Escalante, A Aggarwal C R.]] |
[[Category: Thanos, D.]] | [[Category: Thanos, D.]] | ||
[[Category: Yie, J.]] | [[Category: Yie, J.]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:13 2008'' |
Revision as of 11:11, 21 February 2008
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INTERFERON REGULATORY FACTOR 1 (IRF-1) COMPLEX WITH DNA
Overview
The family of interferon regulatory factor (IRF) transcription factors is important in the regulation of interferons in response to infection by virus and in the regulation of interferon-inducible genes. The IRF family is characterized by a unique 'tryptophan cluster' DNA-binding region. Here we report the crystal structure of the IRF-1 region bound to the natural positive regulatory domain I (PRD I) DNA element from the interferon-beta promoter. The structure provides the first three-dimensional view of a member of the growing IRF family, revealing a new helix-turn-helix motif that latches onto DNA through three of the five conserved tryptophans. The motif selects a short GAAA core sequence through an obliquely angled recognition helix, with an accompanying bending of the DNA axis in the direction of the protein. Together, these features suggest a basis for the occurrence of GAAA repeats within IRF response elements and provide clues to the assembly of the higher-order interferon-beta enhancesome.
About this Structure
1IF1 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure of IRF-1 with bound DNA reveals determinants of interferon regulation., Escalante CR, Yie J, Thanos D, Aggarwal AK, Nature. 1998 Jan 1;391(6662):103-6. PMID:9422515
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