1jbj
From Proteopedia
(New page: 200px<br /><applet load="1jbj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jbj" /> '''CD3 Epsilon and gamma Ectodomain Fragment Co...) |
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| - | [[Image:1jbj.jpg|left|200px]]<br /><applet load="1jbj" size=" | + | [[Image:1jbj.jpg|left|200px]]<br /><applet load="1jbj" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''CD3 Epsilon and gamma Ectodomain Fragment Complex in Single-Chain Construct'''<br /> | '''CD3 Epsilon and gamma Ectodomain Fragment Complex in Single-Chain Construct'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The T cell receptor (TCR) consists of genetically diverse disulfide-linked | + | The T cell receptor (TCR) consists of genetically diverse disulfide-linked alpha and beta chains in noncovalent association with the invariant CD3 subunits. CD3 epsilon and CD3 gamma are integral components of both the TCR and pre-TCR. Here, we present the solution structure of a heterodimeric CD3 epsilon gamma ectodomain complex. A unique side-to-side hydrophobic interface between the two C2-set immunoglobulin-like domains and parallel pairing of their respective C-terminal beta strands are revealed. Mutational analysis confirms the importance of the distinctive linkage as well as the membrane proximal stalk motif (RxCxxCxE) for domain-domain association. These biochemical and structural analyses offer insights into the modular pairwise association of CD3 invariant chains. More importantly, the findings suggest how the rigidified CD3 elements participate in TCR-based signal transduction. |
==About this Structure== | ==About this Structure== | ||
| - | 1JBJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1JBJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JBJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kim, K | + | [[Category: Kim, K S.]] |
| - | [[Category: Reinherz, E | + | [[Category: Reinherz, E L.]] |
| - | [[Category: Sun, Z | + | [[Category: Sun, Z Y.J.]] |
[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
[[Category: beta-sheet]] | [[Category: beta-sheet]] | ||
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[[Category: single-chain]] | [[Category: single-chain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:20:47 2008'' |
Revision as of 11:20, 21 February 2008
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CD3 Epsilon and gamma Ectodomain Fragment Complex in Single-Chain Construct
Overview
The T cell receptor (TCR) consists of genetically diverse disulfide-linked alpha and beta chains in noncovalent association with the invariant CD3 subunits. CD3 epsilon and CD3 gamma are integral components of both the TCR and pre-TCR. Here, we present the solution structure of a heterodimeric CD3 epsilon gamma ectodomain complex. A unique side-to-side hydrophobic interface between the two C2-set immunoglobulin-like domains and parallel pairing of their respective C-terminal beta strands are revealed. Mutational analysis confirms the importance of the distinctive linkage as well as the membrane proximal stalk motif (RxCxxCxE) for domain-domain association. These biochemical and structural analyses offer insights into the modular pairwise association of CD3 invariant chains. More importantly, the findings suggest how the rigidified CD3 elements participate in TCR-based signal transduction.
About this Structure
1JBJ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Mechanisms contributing to T cell receptor signaling and assembly revealed by the solution structure of an ectodomain fragment of the CD3 epsilon gamma heterodimer., Sun ZJ, Kim KS, Wagner G, Reinherz EL, Cell. 2001 Jun 29;105(7):913-23. PMID:11439187
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