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1jeo
From Proteopedia
(New page: 200px<br /><applet load="1jeo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jeo, resolution 2.0Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1jeo.gif|left|200px]]<br /><applet load="1jeo" size=" | + | [[Image:1jeo.gif|left|200px]]<br /><applet load="1jeo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jeo, resolution 2.0Å" /> | caption="1jeo, resolution 2.0Å" /> | ||
'''Crystal Structure of the Hypothetical Protein MJ1247 from Methanococcus jannaschii at 2.0 A Resolution Infers a Molecular Function of 3-Hexulose-6-Phosphate isomerase.'''<br /> | '''Crystal Structure of the Hypothetical Protein MJ1247 from Methanococcus jannaschii at 2.0 A Resolution Infers a Molecular Function of 3-Hexulose-6-Phosphate isomerase.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the hypothetical protein MJ1247 from | + | The crystal structure of the hypothetical protein MJ1247 from Methanococccus jannaschii at 2 A resolution, a detailed sequence analysis, and biochemical assays infer its molecular function to be 3-hexulose-6-phosphate isomerase (PHI). In the dissimilatory ribulose monophosphate (RuMP) cycle, ribulose-5-phosphate is coupled to formaldehyde by the 3-hexulose-6-phosphate synthase (HPS), yielding hexulose-6-phosphate, which is then isomerized to fructose-6-phosphate by the enzyme 3-hexulose-6-phosphate isomerase. MJ1247 is an alpha/beta structure consisting of a five-stranded parallel beta sheet flanked on both sides by alpha helices, forming a three-layered alpha-beta-alpha sandwich. The fold represents the nucleotide binding motif of a flavodoxin type. MJ1247 is a tetramer in the crystal and in solution and each monomer has a folding similar to the isomerase domain of glucosamine-6-phosphate synthase (GlmS). |
==About this Structure== | ==About this Structure== | ||
| - | 1JEO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with CIT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JEO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JEO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: BSGC, Berkeley | + | [[Category: BSGC, Berkeley Structural Genomics Center.]] |
| - | [[Category: Boisvert, D | + | [[Category: Boisvert, D C.]] |
| - | [[Category: Dreyer, M | + | [[Category: Dreyer, M K.]] |
[[Category: Kim, R.]] | [[Category: Kim, R.]] | ||
| - | [[Category: Kim, S | + | [[Category: Kim, S H.]] |
| - | [[Category: Martinez-Chantar, M | + | [[Category: Martinez-Chantar, M L.]] |
| - | [[Category: Martinez-Cruz, L | + | [[Category: Martinez-Cruz, L A.]] |
[[Category: Yokota, H.]] | [[Category: Yokota, H.]] | ||
[[Category: CIT]] | [[Category: CIT]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:48 2008'' |
Revision as of 11:21, 21 February 2008
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Crystal Structure of the Hypothetical Protein MJ1247 from Methanococcus jannaschii at 2.0 A Resolution Infers a Molecular Function of 3-Hexulose-6-Phosphate isomerase.
Overview
The crystal structure of the hypothetical protein MJ1247 from Methanococccus jannaschii at 2 A resolution, a detailed sequence analysis, and biochemical assays infer its molecular function to be 3-hexulose-6-phosphate isomerase (PHI). In the dissimilatory ribulose monophosphate (RuMP) cycle, ribulose-5-phosphate is coupled to formaldehyde by the 3-hexulose-6-phosphate synthase (HPS), yielding hexulose-6-phosphate, which is then isomerized to fructose-6-phosphate by the enzyme 3-hexulose-6-phosphate isomerase. MJ1247 is an alpha/beta structure consisting of a five-stranded parallel beta sheet flanked on both sides by alpha helices, forming a three-layered alpha-beta-alpha sandwich. The fold represents the nucleotide binding motif of a flavodoxin type. MJ1247 is a tetramer in the crystal and in solution and each monomer has a folding similar to the isomerase domain of glucosamine-6-phosphate synthase (GlmS).
About this Structure
1JEO is a Single protein structure of sequence from Methanocaldococcus jannaschii with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of MJ1247 protein from M. jannaschii at 2.0 A resolution infers a molecular function of 3-hexulose-6-phosphate isomerase., Martinez-Cruz LA, Dreyer MK, Boisvert DC, Yokota H, Martinez-Chantar ML, Kim R, Kim SH, Structure. 2002 Feb;10(2):195-204. PMID:11839305
Page seeded by OCA on Thu Feb 21 13:21:48 2008
Categories: Methanocaldococcus jannaschii | Single protein | BSGC, Berkeley Structural Genomics Center. | Boisvert, D C. | Dreyer, M K. | Kim, R. | Kim, S H. | Martinez-Chantar, M L. | Martinez-Cruz, L A. | Yokota, H. | CIT | 3-hexulose-6-phosphate isomerase | Berkeley structural genomics center | Bsgc structure funded by nih | Phi | Phosphosugar | Protein structure initiative | Psi | Rump pathway | Structural genomics
