1jhi
From Proteopedia
(New page: 200px<br /><applet load="1jhi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jhi" /> '''Solution Structure of a Hedamycin-DNA comple...) |
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| - | [[Image:1jhi.gif|left|200px]]<br /><applet load="1jhi" size=" | + | [[Image:1jhi.gif|left|200px]]<br /><applet load="1jhi" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jhi" /> | caption="1jhi" /> | ||
'''Solution Structure of a Hedamycin-DNA complex'''<br /> | '''Solution Structure of a Hedamycin-DNA complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Hedamycin, a member of the pluramycin family of drugs, displays a range of | + | Hedamycin, a member of the pluramycin family of drugs, displays a range of biological responses including antitumor and antimicrobial activity. The mechanism of action is via direct interaction with DNA through intercalation between the bases of the oligonucleotide and alkylation of a guanine residue at 5'-PyG-3' sites. There appears to be some minor structural differences between two earlier studies on the interaction of hedamycin with 5'-PyG-3' sites. In this study, a high-resolution NMR analysis of the hedamycin:d(ACCGGT)2 complex was undertaken in order to investigate the effect of replacing the thymine with a guanine at the preferred 5'-CGT-3' site. The resultant structure was compared with earlier work, with particular emphasis placed on the drug conformation. The structure of the hedamycin:d(ACCGGT)2 complex has many features in common with the two previous NMR structures of hedamycin:DNA complexes but differed in the conformation and orientation of the N,N-dimethylvancosamine saccharide of hedamycin in one of these structures. The preferential binding of hedamycin to 5'-CG-3' over 5'-TG-3' binding sites is explained in terms of the orientation and location of the N,N-dimethylvancosamine saccharide in the minor groove. |
==About this Structure== | ==About this Structure== | ||
| - | 1JHI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with HEH as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1JHI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=HEH:'>HEH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHI OCA]. |
==Reference== | ==Reference== | ||
Structural investigation of the hedamycin:d(ACCGGT)2 complex by NMR and restrained molecular dynamics., Owen EA, Burley GA, Carver JA, Wickham G, Keniry MA, Biochem Biophys Res Commun. 2002 Feb 8;290(5):1602-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11820806 11820806] | Structural investigation of the hedamycin:d(ACCGGT)2 complex by NMR and restrained molecular dynamics., Owen EA, Burley GA, Carver JA, Wickham G, Keniry MA, Biochem Biophys Res Commun. 2002 Feb 8;290(5):1602-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11820806 11820806] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Burley, G | + | [[Category: Burley, G A.]] |
| - | [[Category: Carver, J | + | [[Category: Carver, J A.]] |
| - | [[Category: Keniry, M | + | [[Category: Keniry, M A.]] |
| - | [[Category: Owen, E | + | [[Category: Owen, E A.]] |
[[Category: Wickham, G.]] | [[Category: Wickham, G.]] | ||
[[Category: HEH]] | [[Category: HEH]] | ||
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[[Category: hedamycin]] | [[Category: hedamycin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:41 2008'' |
Revision as of 11:22, 21 February 2008
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Solution Structure of a Hedamycin-DNA complex
Overview
Hedamycin, a member of the pluramycin family of drugs, displays a range of biological responses including antitumor and antimicrobial activity. The mechanism of action is via direct interaction with DNA through intercalation between the bases of the oligonucleotide and alkylation of a guanine residue at 5'-PyG-3' sites. There appears to be some minor structural differences between two earlier studies on the interaction of hedamycin with 5'-PyG-3' sites. In this study, a high-resolution NMR analysis of the hedamycin:d(ACCGGT)2 complex was undertaken in order to investigate the effect of replacing the thymine with a guanine at the preferred 5'-CGT-3' site. The resultant structure was compared with earlier work, with particular emphasis placed on the drug conformation. The structure of the hedamycin:d(ACCGGT)2 complex has many features in common with the two previous NMR structures of hedamycin:DNA complexes but differed in the conformation and orientation of the N,N-dimethylvancosamine saccharide of hedamycin in one of these structures. The preferential binding of hedamycin to 5'-CG-3' over 5'-TG-3' binding sites is explained in terms of the orientation and location of the N,N-dimethylvancosamine saccharide in the minor groove.
About this Structure
1JHI is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structural investigation of the hedamycin:d(ACCGGT)2 complex by NMR and restrained molecular dynamics., Owen EA, Burley GA, Carver JA, Wickham G, Keniry MA, Biochem Biophys Res Commun. 2002 Feb 8;290(5):1602-8. PMID:11820806
Page seeded by OCA on Thu Feb 21 13:22:41 2008
Categories: Protein complex | Burley, G A. | Carver, J A. | Keniry, M A. | Owen, E A. | Wickham, G. | HEH | Duplex dna | Hedamycin
