1jjh
From Proteopedia
(New page: 200px<br /><applet load="1jjh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jjh, resolution 2.50Å" /> '''E2 DNA-binding Domai...) |
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| - | [[Image:1jjh.gif|left|200px]]<br /><applet load="1jjh" size=" | + | [[Image:1jjh.gif|left|200px]]<br /><applet load="1jjh" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jjh, resolution 2.50Å" /> | caption="1jjh, resolution 2.50Å" /> | ||
'''E2 DNA-binding Domain from Bovine Papillomavirus Type 1'''<br /> | '''E2 DNA-binding Domain from Bovine Papillomavirus Type 1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 2.5 A crystal structures of the DNA-binding domain of the E2 protein | + | The 2.5 A crystal structures of the DNA-binding domain of the E2 protein from bovine papillomavirus strain 1 and its complex with DNA are presented. E2 is a transcriptional regulatory protein that is also involved in viral DNA replication. It is the structural prototype for a novel class of DNA-binding proteins: dimeric beta-barrels with surface alpha-helices that serve as recognition helices. These helices contain the amino-acid residues involved in sequence-specifying interactions. The E2 proteins from different papillomavirus strains recognize and bind to the same consensus 12 base-pair DNA sequence. However, recent evidence from solution studies points to differences in the mechanisms by which E2 from the related viral strains bovine papillomavirus-1 and human papillomavirus-16 discriminate between DNA targets based on non-contacted nucleotide sequences. This report provides evidence that sequence-specific DNA-binding is accompanied by a rearrangement of protein subunits and deformation of the DNA. These results suggest that, along with DNA sequence-dependent conformational properties, protein subunit orientation plays a significant role in the mechanisms of target selection utilized by E2. |
==About this Structure== | ==About this Structure== | ||
| - | 1JJH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus_type_1 Bovine papillomavirus type 1]. Full crystallographic information is available from [http:// | + | 1JJH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus_type_1 Bovine papillomavirus type 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bovine papillomavirus type 1]] | [[Category: Bovine papillomavirus type 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hegde, R | + | [[Category: Hegde, R S.]] |
| - | [[Category: Kim, S | + | [[Category: Kim, S S.]] |
[[Category: Schapira, M.]] | [[Category: Schapira, M.]] | ||
| - | [[Category: Wang, A | + | [[Category: Wang, A F.]] |
[[Category: bpv-1]] | [[Category: bpv-1]] | ||
[[Category: dna-binding domain]] | [[Category: dna-binding domain]] | ||
[[Category: e2]] | [[Category: e2]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:24 2008'' |
Revision as of 11:23, 21 February 2008
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E2 DNA-binding Domain from Bovine Papillomavirus Type 1
Overview
The 2.5 A crystal structures of the DNA-binding domain of the E2 protein from bovine papillomavirus strain 1 and its complex with DNA are presented. E2 is a transcriptional regulatory protein that is also involved in viral DNA replication. It is the structural prototype for a novel class of DNA-binding proteins: dimeric beta-barrels with surface alpha-helices that serve as recognition helices. These helices contain the amino-acid residues involved in sequence-specifying interactions. The E2 proteins from different papillomavirus strains recognize and bind to the same consensus 12 base-pair DNA sequence. However, recent evidence from solution studies points to differences in the mechanisms by which E2 from the related viral strains bovine papillomavirus-1 and human papillomavirus-16 discriminate between DNA targets based on non-contacted nucleotide sequences. This report provides evidence that sequence-specific DNA-binding is accompanied by a rearrangement of protein subunits and deformation of the DNA. These results suggest that, along with DNA sequence-dependent conformational properties, protein subunit orientation plays a significant role in the mechanisms of target selection utilized by E2.
About this Structure
1JJH is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.
Reference
Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein., Hegde RS, Wang AF, Kim SS, Schapira M, J Mol Biol. 1998 Mar 6;276(4):797-808. PMID:9500927
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