1jky
From Proteopedia
(New page: 200px<br /> <applet load="1jky" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jky, resolution 3.90Å" /> '''Crystal Structure o...) |
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caption="1jky, resolution 3.90Å" /> | caption="1jky, resolution 3.90Å" /> | ||
'''Crystal Structure of the Anthrax Lethal Factor (LF): Wild-type LF Complexed with the N-terminal Sequence of MAPKK2'''<br /> | '''Crystal Structure of the Anthrax Lethal Factor (LF): Wild-type LF Complexed with the N-terminal Sequence of MAPKK2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Lethal factor (LF) is a protein (relative molecular mass 90,000) that is | + | Lethal factor (LF) is a protein (relative molecular mass 90,000) that is critical in the pathogenesis of anthrax. It is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near to their amino termini, leading to the inhibition of one or more signalling pathways. Here we describe the crystal structure of LF and its complex with the N terminus of MAPKK-2. LF comprises four domains: domain I binds the membrane-translocating component of anthrax toxin, the protective antigen (PA); domains II, III and IV together create a long deep groove that holds the 16-residue N-terminal tail of MAPKK-2 before cleavage. Domain II resembles the ADP-ribosylating toxin from Bacillus cereus, but the active site has been mutated and recruited to augment substrate recognition. Domain III is inserted into domain II, and seems to have arisen from a repeated duplication of a structural element of domain II. Domain IV is distantly related to the zinc metalloprotease family, and contains the catalytic centre; it also resembles domain I. The structure thus reveals a protein that has evolved through a process of gene duplication, mutation and fusion, into an enzyme with high and unusual specificity. |
==About this Structure== | ==About this Structure== | ||
| - | 1JKY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. The following page contains interesting information on the relation of 1JKY with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb28_1.html Anthrax Toxin]]. Full crystallographic information is available from [http:// | + | 1JKY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. The following page contains interesting information on the relation of 1JKY with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb28_1.html Anthrax Toxin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bienkowska, J.]] | [[Category: Bienkowska, J.]] | ||
| - | [[Category: Collier, R | + | [[Category: Collier, R J.]] |
[[Category: Hanna, P.]] | [[Category: Hanna, P.]] | ||
| - | [[Category: Lacy, D | + | [[Category: Lacy, D B.]] |
| - | [[Category: Leppla, S | + | [[Category: Leppla, S H.]] |
| - | [[Category: Liddington, R | + | [[Category: Liddington, R C.]] |
| - | [[Category: Pannifer, A | + | [[Category: Pannifer, A D.]] |
[[Category: Park, S.]] | [[Category: Park, S.]] | ||
[[Category: Petosa,C.]] | [[Category: Petosa,C.]] | ||
[[Category: Renatus, M.]] | [[Category: Renatus, M.]] | ||
[[Category: Schwarzenbacher, R.]] | [[Category: Schwarzenbacher, R.]] | ||
| - | [[Category: Wong, T | + | [[Category: Wong, T Y.]] |
[[Category: lethal toxin]] | [[Category: lethal toxin]] | ||
[[Category: mapkk]] | [[Category: mapkk]] | ||
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[[Category: uncleaved substrate]] | [[Category: uncleaved substrate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:49 2008'' |
Revision as of 11:23, 21 February 2008
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Crystal Structure of the Anthrax Lethal Factor (LF): Wild-type LF Complexed with the N-terminal Sequence of MAPKK2
Overview
Lethal factor (LF) is a protein (relative molecular mass 90,000) that is critical in the pathogenesis of anthrax. It is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near to their amino termini, leading to the inhibition of one or more signalling pathways. Here we describe the crystal structure of LF and its complex with the N terminus of MAPKK-2. LF comprises four domains: domain I binds the membrane-translocating component of anthrax toxin, the protective antigen (PA); domains II, III and IV together create a long deep groove that holds the 16-residue N-terminal tail of MAPKK-2 before cleavage. Domain II resembles the ADP-ribosylating toxin from Bacillus cereus, but the active site has been mutated and recruited to augment substrate recognition. Domain III is inserted into domain II, and seems to have arisen from a repeated duplication of a structural element of domain II. Domain IV is distantly related to the zinc metalloprotease family, and contains the catalytic centre; it also resembles domain I. The structure thus reveals a protein that has evolved through a process of gene duplication, mutation and fusion, into an enzyme with high and unusual specificity.
About this Structure
1JKY is a Protein complex structure of sequences from Bacillus anthracis. The following page contains interesting information on the relation of 1JKY with [Anthrax Toxin]. Full crystallographic information is available from OCA.
Reference
Crystal structure of the anthrax lethal factor., Pannifer AD, Wong TY, Schwarzenbacher R, Renatus M, Petosa C, Bienkowska J, Lacy DB, Collier RJ, Park S, Leppla SH, Hanna P, Liddington RC, Nature. 2001 Nov 8;414(6860):229-33. PMID:11700563
Page seeded by OCA on Thu Feb 21 13:23:49 2008
Categories: Anthrax Toxin | Bacillus anthracis | Protein complex | Bienkowska, J. | Collier, R J. | Hanna, P. | Lacy, D B. | Leppla, S H. | Liddington, R C. | Pannifer, A D. | Park, S. | Petosa,C. | Renatus, M. | Schwarzenbacher, R. | Wong, T Y. | Lethal toxin | Mapkk | Mek2 | Protease-substrate complex | Uncleaved substrate
