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1jxa
From Proteopedia
(New page: 200px<br /><applet load="1jxa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jxa, resolution 3.1Å" /> '''GLUCOSAMINE 6-PHOSPHA...) |
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| - | [[Image:1jxa.gif|left|200px]]<br /><applet load="1jxa" size=" | + | [[Image:1jxa.gif|left|200px]]<br /><applet load="1jxa" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jxa, resolution 3.1Å" /> | caption="1jxa, resolution 3.1Å" /> | ||
'''GLUCOSAMINE 6-PHOSPHATE SYNTHASE WITH GLUCOSE 6-PHOSPHATE'''<br /> | '''GLUCOSAMINE 6-PHOSPHATE SYNTHASE WITH GLUCOSE 6-PHOSPHATE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Glucosamine-6-phosphate synthase catalyses the first and rate-limiting | + | Glucosamine-6-phosphate synthase catalyses the first and rate-limiting step in hexosamine metabolism, converting fructose 6-phosphate into glucosamine 6-phosphate in the presence of glutamine. The crystal structure of the Escherichia coli enzyme reveals the domain organisation of the homodimeric molecule. The 18 A hydrophobic channel sequestered from the solvent connects the glutaminase and isomerase active sites, and provides a means of ammonia transfer from glutamine to sugar phosphate. The C-terminal decapeptide sandwiched between the two domains plays a central role in the transfer. Based on the structure, a mechanism of enzyme action and self-regulation is proposed. It involves large domain movements triggered by substrate binding that lead to the formation of the channel. |
==About this Structure== | ==About this Structure== | ||
| - | 1JXA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with G6Q as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutamine--fructose-6-phosphate_transaminase_(isomerizing) Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16] Full crystallographic information is available from [http:// | + | 1JXA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=G6Q:'>G6Q</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutamine--fructose-6-phosphate_transaminase_(isomerizing) Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Badet, B.]] | [[Category: Badet, B.]] | ||
| - | [[Category: Badet-Denisot, M | + | [[Category: Badet-Denisot, M A.]] |
[[Category: Obmolova, G.]] | [[Category: Obmolova, G.]] | ||
[[Category: Teplyakov, A.]] | [[Category: Teplyakov, A.]] | ||
| Line 24: | Line 24: | ||
[[Category: nucleotide-binding fold]] | [[Category: nucleotide-binding fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:45 2008'' |
Revision as of 11:27, 21 February 2008
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GLUCOSAMINE 6-PHOSPHATE SYNTHASE WITH GLUCOSE 6-PHOSPHATE
Overview
Glucosamine-6-phosphate synthase catalyses the first and rate-limiting step in hexosamine metabolism, converting fructose 6-phosphate into glucosamine 6-phosphate in the presence of glutamine. The crystal structure of the Escherichia coli enzyme reveals the domain organisation of the homodimeric molecule. The 18 A hydrophobic channel sequestered from the solvent connects the glutaminase and isomerase active sites, and provides a means of ammonia transfer from glutamine to sugar phosphate. The C-terminal decapeptide sandwiched between the two domains plays a central role in the transfer. Based on the structure, a mechanism of enzyme action and self-regulation is proposed. It involves large domain movements triggered by substrate binding that lead to the formation of the channel.
About this Structure
1JXA is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Glutamine--fructose-6-phosphate transaminase (isomerizing), with EC number 2.6.1.16 Full crystallographic information is available from OCA.
Reference
Channeling of ammonia in glucosamine-6-phosphate synthase., Teplyakov A, Obmolova G, Badet B, Badet-Denisot MA, J Mol Biol. 2001 Nov 9;313(5):1093-102. PMID:11700065
Page seeded by OCA on Thu Feb 21 13:27:45 2008
