This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1k4u
From Proteopedia
(New page: 200px<br /> <applet load="1k4u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k4u" /> '''Solution structure of the C-terminal SH3 do...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1k4u.gif|left|200px]]<br /> | + | [[Image:1k4u.gif|left|200px]]<br /><applet load="1k4u" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1k4u" size=" | + | |
caption="1k4u" /> | caption="1k4u" /> | ||
'''Solution structure of the C-terminal SH3 domain of p67phox complexed with the C-terminal tail region of p47phox'''<br /> | '''Solution structure of the C-terminal SH3 domain of p67phox complexed with the C-terminal tail region of p47phox'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The basic function of the Src homology 3 (SH3) domain is considered to be | + | The basic function of the Src homology 3 (SH3) domain is considered to be binding to proline-rich sequences containing a PxxP motif. Recently, many SH3 domains, including those from Grb2 and Pex13p, were reported to bind sequences lacking a PxxP motif. We report here that the 22 residue peptide lacking a PxxP motif, derived from p47(phox), binds to the C-terminal SH3 domain from p67(phox). We applied the NMR cross-saturation method to locate the interaction sites for the non-PxxP peptides on their cognate SH3 domains from p67(phox), Grb2 and Pex13p. The binding site of the Grb2 SH3 partially overlapped the conventional PxxP-binding site, whereas those of p67(phox) and Pex13p SH3s are located in different surface regions. The non-PxxP peptide from p47(phox) binds to the p67(phox) SH3 more tightly when it extends to the N-terminus to include a typical PxxP motif, which enabled the structure determination of the complex, to reveal that the non-PxxP peptide segment interacted with the p67(phox) SH3 in a compact helix-turn-helix structure (PDB entry 1K4U). |
==Disease== | ==Disease== | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1K4U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1K4U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K4U OCA]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 25: | ||
[[Category: sh3-peptide complex]] | [[Category: sh3-peptide complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:09 2008'' |
Revision as of 11:30, 21 February 2008
|
Solution structure of the C-terminal SH3 domain of p67phox complexed with the C-terminal tail region of p47phox
Contents |
Overview
The basic function of the Src homology 3 (SH3) domain is considered to be binding to proline-rich sequences containing a PxxP motif. Recently, many SH3 domains, including those from Grb2 and Pex13p, were reported to bind sequences lacking a PxxP motif. We report here that the 22 residue peptide lacking a PxxP motif, derived from p47(phox), binds to the C-terminal SH3 domain from p67(phox). We applied the NMR cross-saturation method to locate the interaction sites for the non-PxxP peptides on their cognate SH3 domains from p67(phox), Grb2 and Pex13p. The binding site of the Grb2 SH3 partially overlapped the conventional PxxP-binding site, whereas those of p67(phox) and Pex13p SH3s are located in different surface regions. The non-PxxP peptide from p47(phox) binds to the p67(phox) SH3 more tightly when it extends to the N-terminus to include a typical PxxP motif, which enabled the structure determination of the complex, to reveal that the non-PxxP peptide segment interacted with the p67(phox) SH3 in a compact helix-turn-helix structure (PDB entry 1K4U).
Disease
Known diseases associated with this structure: Chronic granulomatous disease due to deficiency of NCF-1 OMIM:[608512], Chronic granulomatous disease due to deficiency of NCF-2 OMIM:[608515]
About this Structure
1K4U is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Diverse recognition of non-PxxP peptide ligands by the SH3 domains from p67(phox), Grb2 and Pex13p., Kami K, Takeya R, Sumimoto H, Kohda D, EMBO J. 2002 Aug 15;21(16):4268-76. PMID:12169629
Page seeded by OCA on Thu Feb 21 13:30:09 2008
