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1k5w
From Proteopedia
(New page: 200px<br /><applet load="1k5w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k5w" /> '''THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTA...) |
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| - | [[Image:1k5w.jpg|left|200px]]<br /><applet load="1k5w" size=" | + | [[Image:1k5w.jpg|left|200px]]<br /><applet load="1k5w" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k5w" /> | caption="1k5w" /> | ||
'''THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE'''<br /> | '''THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Synaptotagmin 1 probably functions as a Ca2+ sensor in neurotransmitter | + | Synaptotagmin 1 probably functions as a Ca2+ sensor in neurotransmitter release via its two C2-domains, but no common Ca2+-dependent activity that could underlie a cooperative action between them has been described. The NMR structure of the C2B-domain now reveals a beta sandwich that exhibits striking similarities and differences with the C2A-domain. Whereas the bottom face of the C2B-domain has two additional alpha helices that may be involved in specialized Ca2+-independent functions, the top face binds two Ca2+ ions and is remarkably similar to the C2A-domain. Consistent with these results, but in contrast to previous studies, we find that the C2B-domain binds phospholipids in a Ca2+-dependent manner similarly to the C2A-domain. These results suggest a novel view of synaptotagmin function whereby the two C2-domains cooperate in a common activity, Ca2+-dependent phospholipid binding, to trigger neurotransmitter release. |
==About this Structure== | ==About this Structure== | ||
| - | 1K5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1K5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5W OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Anderson, R | + | [[Category: Anderson, R G.W.]] |
[[Category: Arac, D.]] | [[Category: Arac, D.]] | ||
[[Category: Fernandez, I.]] | [[Category: Fernandez, I.]] | ||
[[Category: Gao, Y.]] | [[Category: Gao, Y.]] | ||
| - | [[Category: Gerber, S | + | [[Category: Gerber, S H.]] |
[[Category: Rizo, J.]] | [[Category: Rizo, J.]] | ||
[[Category: Shin, O.]] | [[Category: Shin, O.]] | ||
| - | [[Category: Sudhof, T | + | [[Category: Sudhof, T C.]] |
[[Category: Ubach, J.]] | [[Category: Ubach, J.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
| Line 32: | Line 32: | ||
[[Category: synaptotagmin i]] | [[Category: synaptotagmin i]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:31 2008'' |
Revision as of 11:30, 21 February 2008
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THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE
Overview
Synaptotagmin 1 probably functions as a Ca2+ sensor in neurotransmitter release via its two C2-domains, but no common Ca2+-dependent activity that could underlie a cooperative action between them has been described. The NMR structure of the C2B-domain now reveals a beta sandwich that exhibits striking similarities and differences with the C2A-domain. Whereas the bottom face of the C2B-domain has two additional alpha helices that may be involved in specialized Ca2+-independent functions, the top face binds two Ca2+ ions and is remarkably similar to the C2A-domain. Consistent with these results, but in contrast to previous studies, we find that the C2B-domain binds phospholipids in a Ca2+-dependent manner similarly to the C2A-domain. These results suggest a novel view of synaptotagmin function whereby the two C2-domains cooperate in a common activity, Ca2+-dependent phospholipid binding, to trigger neurotransmitter release.
About this Structure
1K5W is a Single protein structure of sequence from Rattus norvegicus with as ligand. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine., Fernandez I, Arac D, Ubach J, Gerber SH, Shin O, Gao Y, Anderson RG, Sudhof TC, Rizo J, Neuron. 2001 Dec 20;32(6):1057-69. PMID:11754837
Page seeded by OCA on Thu Feb 21 13:30:31 2008
Categories: Rattus norvegicus | Single protein | Anderson, R G.W. | Arac, D. | Fernandez, I. | Gao, Y. | Gerber, S H. | Rizo, J. | Shin, O. | Sudhof, T C. | Ubach, J. | CA | C2-domain | C2b-domain | Calcium-binding | Neurotransmitter release | Phospholipid-binding | Synapsis | Synaptic vesicle exocytosis | Synaptotagmin i
