1khp
From Proteopedia
(New page: 200px<br /><applet load="1khp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1khp, resolution 2.0Å" /> '''Monoclinic form of pa...) |
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| - | [[Image:1khp.jpg|left|200px]]<br /><applet load="1khp" size=" | + | [[Image:1khp.jpg|left|200px]]<br /><applet load="1khp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1khp, resolution 2.0Å" /> | caption="1khp, resolution 2.0Å" /> | ||
'''Monoclinic form of papain/ZLFG-DAM covalent complex'''<br /> | '''Monoclinic form of papain/ZLFG-DAM covalent complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of two polymorphs of a ZLFG-CH2-papain | + | The three-dimensional structure of two polymorphs of a ZLFG-CH2-papain covalent complex has been determined by X-ray crystallography. The structures indicate that: (i) the methylene carbon atom of the inhibitor is covalently bound to the Sgamma atom of Cys25 of papain; (ii) the hydrophobic S2 pocket formed by Pro68, Val133, Val157, and Asp158 is occupied by the inhibitor's phenylalanyl P2 side chain; (iii) extensive hydrogen bonding and hydrophobic interactions are responsible for the interaction of the inhibitor with the enzyme. Comparison with similar structures suggests that in covalent complexes preservation of main chain-main chain interactions between the enzyme and the inhibitor may have higher priority than the P-S interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 1KHP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Active as [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] Full crystallographic information is available from [http:// | + | 1KHP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Active as [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protease inhibitor]] | [[Category: protease inhibitor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:17 2008'' |
Revision as of 11:34, 21 February 2008
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Monoclinic form of papain/ZLFG-DAM covalent complex
Overview
The three-dimensional structure of two polymorphs of a ZLFG-CH2-papain covalent complex has been determined by X-ray crystallography. The structures indicate that: (i) the methylene carbon atom of the inhibitor is covalently bound to the Sgamma atom of Cys25 of papain; (ii) the hydrophobic S2 pocket formed by Pro68, Val133, Val157, and Asp158 is occupied by the inhibitor's phenylalanyl P2 side chain; (iii) extensive hydrogen bonding and hydrophobic interactions are responsible for the interaction of the inhibitor with the enzyme. Comparison with similar structures suggests that in covalent complexes preservation of main chain-main chain interactions between the enzyme and the inhibitor may have higher priority than the P-S interactions.
About this Structure
1KHP is a Single protein structure of sequence from Carica papaya. Active as Papain, with EC number 3.4.22.2 Full crystallographic information is available from OCA.
Reference
Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor., Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M, J Pept Res. 2004 Oct;64(4):141-50. PMID:15357669
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