1ksh
From Proteopedia
(New page: 200px<br /> <applet load="1ksh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ksh, resolution 1.80Å" /> '''Complex of Arl2 and...) |
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| - | [[Image:1ksh.gif|left|200px]]<br /> | + | [[Image:1ksh.gif|left|200px]]<br /><applet load="1ksh" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ksh" size=" | + | |
caption="1ksh, resolution 1.80Å" /> | caption="1ksh, resolution 1.80Å" /> | ||
'''Complex of Arl2 and PDE delta, Crystal Form 2 (native)'''<br /> | '''Complex of Arl2 and PDE delta, Crystal Form 2 (native)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Arf-like (Arl) proteins are close relatives of the Arf regulators of | + | Arf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with its effector PDE delta solved in two crystal forms (Protein Data Bank codes 1KSG, 1KSH and 1KSJ). Arl2 shows a dramatic conformational change from the GDP-bound form, which suggests that it is reversibly membrane associated. PDE delta is structurally closely related to RhoGDI and contains a deep empty hydrophobic pocket. Further experiments show that H-Ras, Rheb, Rho6 and G alpha(i1) interact with PDE delta and that, at least for H-Ras, the intact C-terminus is required. We suggest PDE delta to be a specific soluble transport factor for certain prenylated proteins and Arl2-GTP a regulator of PDE delta-mediated transport. |
==About this Structure== | ==About this Structure== | ||
| - | 1KSH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG, PO4 and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3',5'-cyclic-nucleotide_phosphodiesterase 3',5'-cyclic-nucleotide phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.17 3.1.4.17] Full crystallographic information is available from [http:// | + | 1KSH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3',5'-cyclic-nucleotide_phosphodiesterase 3',5'-cyclic-nucleotide phosphodiesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.17 3.1.4.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Hanzal-Bayer, M.]] | [[Category: Hanzal-Bayer, M.]] | ||
| - | [[Category: Hillig, R | + | [[Category: Hillig, R C.]] |
[[Category: Renault, L.]] | [[Category: Renault, L.]] | ||
[[Category: Roversi, P.]] | [[Category: Roversi, P.]] | ||
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[[Category: small gtpase]] | [[Category: small gtpase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:37:28 2008'' |
Revision as of 11:37, 21 February 2008
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Complex of Arl2 and PDE delta, Crystal Form 2 (native)
Overview
Arf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with its effector PDE delta solved in two crystal forms (Protein Data Bank codes 1KSG, 1KSH and 1KSJ). Arl2 shows a dramatic conformational change from the GDP-bound form, which suggests that it is reversibly membrane associated. PDE delta is structurally closely related to RhoGDI and contains a deep empty hydrophobic pocket. Further experiments show that H-Ras, Rheb, Rho6 and G alpha(i1) interact with PDE delta and that, at least for H-Ras, the intact C-terminus is required. We suggest PDE delta to be a specific soluble transport factor for certain prenylated proteins and Arl2-GTP a regulator of PDE delta-mediated transport.
About this Structure
1KSH is a Protein complex structure of sequences from Homo sapiens and Mus musculus with , and as ligands. Active as 3',5'-cyclic-nucleotide phosphodiesterase, with EC number 3.1.4.17 Full crystallographic information is available from OCA.
Reference
The complex of Arl2-GTP and PDE delta: from structure to function., Hanzal-Bayer M, Renault L, Roversi P, Wittinghofer A, Hillig RC, EMBO J. 2002 May 1;21(9):2095-106. PMID:11980706
Page seeded by OCA on Thu Feb 21 13:37:28 2008
Categories: 3',5'-cyclic-nucleotide phosphodiesterase | Homo sapiens | Mus musculus | Protein complex | Hanzal-Bayer, M. | Hillig, R C. | Renault, L. | Roversi, P. | Wittinghofer, A. | GDP | MG | PO4 | Arf family | Effector molecule | Gdi | Immunoglobuline-like fold | Small gtp-binding protein | Small gtpase
