1kt2
From Proteopedia
(New page: 200px<br /><applet load="1kt2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kt2, resolution 2.80Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1kt2.jpg|left|200px]]<br /><applet load="1kt2" size=" | + | [[Image:1kt2.jpg|left|200px]]<br /><applet load="1kt2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kt2, resolution 2.80Å" /> | caption="1kt2, resolution 2.80Å" /> | ||
'''CRYSTAL STRUCTURE OF CLASS II MHC MOLECULE IEK BOUND TO MOTH CYTOCHROME C PEPTIDE'''<br /> | '''CRYSTAL STRUCTURE OF CLASS II MHC MOLECULE IEK BOUND TO MOTH CYTOCHROME C PEPTIDE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The COOH-terminal peptides of pigeon and moth cytochrome c, bound to mouse | + | The COOH-terminal peptides of pigeon and moth cytochrome c, bound to mouse IE(k), are two of the most thoroughly studied T cell antigens. We have solved the crystal structures of the moth peptide and a weak agonist-antagonist variant of the pigeon peptide bound to IE(k). The moth peptide and all other peptides whose structures have been solved bound to IE(k), have a lysine filling the p9 pocket of IE(k). However, the pigeon peptide has an alanine at p9 shifting the lysine to p10. Rather than kinking to place the lysine in the anchor pocket, the pigeon peptide takes the extended course through the binding groove, which is characteristic of all other peptides bound to major histocompatibility complex (MHC) class II. Thus, unlike MHC class I, in which peptides often kink to place optimally anchoring side chains, MHC class II imposes an extended peptide conformation even at the cost of a highly conserved anchor residue. The substitution of Ser for Thr at p8 in the variant pigeon peptide induces no detectable surface change other than the loss of the side chain methyl group, despite the dramatic change in recognition by T cells. Finally, these structures can be used to interpret the many published mutational studies of these ligands and the T cell receptors that recognize them. |
==About this Structure== | ==About this Structure== | ||
| - | 1KT2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Moth_and_mus_musculus Moth and mus musculus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1KT2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Moth_and_mus_musculus Moth and mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KT2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Crawford, F.]] | [[Category: Crawford, F.]] | ||
[[Category: Dai, S.]] | [[Category: Dai, S.]] | ||
| - | [[Category: Fremont, D | + | [[Category: Fremont, D H.]] |
[[Category: Kappler, J.]] | [[Category: Kappler, J.]] | ||
[[Category: Marrack, P.]] | [[Category: Marrack, P.]] | ||
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[[Category: t cell receptor]] | [[Category: t cell receptor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:37:39 2008'' |
Revision as of 11:37, 21 February 2008
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CRYSTAL STRUCTURE OF CLASS II MHC MOLECULE IEK BOUND TO MOTH CYTOCHROME C PEPTIDE
Overview
The COOH-terminal peptides of pigeon and moth cytochrome c, bound to mouse IE(k), are two of the most thoroughly studied T cell antigens. We have solved the crystal structures of the moth peptide and a weak agonist-antagonist variant of the pigeon peptide bound to IE(k). The moth peptide and all other peptides whose structures have been solved bound to IE(k), have a lysine filling the p9 pocket of IE(k). However, the pigeon peptide has an alanine at p9 shifting the lysine to p10. Rather than kinking to place the lysine in the anchor pocket, the pigeon peptide takes the extended course through the binding groove, which is characteristic of all other peptides bound to major histocompatibility complex (MHC) class II. Thus, unlike MHC class I, in which peptides often kink to place optimally anchoring side chains, MHC class II imposes an extended peptide conformation even at the cost of a highly conserved anchor residue. The substitution of Ser for Thr at p8 in the variant pigeon peptide induces no detectable surface change other than the loss of the side chain methyl group, despite the dramatic change in recognition by T cells. Finally, these structures can be used to interpret the many published mutational studies of these ligands and the T cell receptors that recognize them.
About this Structure
1KT2 is a Protein complex structure of sequences from Mus musculus and Moth and mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of cytochrome c presentation by IE(k)., Fremont DH, Dai S, Chiang H, Crawford F, Marrack P, Kappler J, J Exp Med. 2002 Apr 15;195(8):1043-52. PMID:11956295
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