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1oc2
From Proteopedia
(New page: 200px<br /> <applet load="1oc2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oc2, resolution 1.50Å" /> '''THE STRUCTURE OF NA...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1OC2 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptococcus_suis Streptococcus suis]] with SO4, TDX and NAD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.46 4.2.1.46]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OC2 OCA]]. | + | 1OC2 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptococcus_suis Streptococcus suis]] with SO4, TDX and NAD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/dTDP-glucose_4,6-dehydratase dTDP-glucose 4,6-dehydratase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.46 4.2.1.46]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OC2 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptococcus suis]] | [[Category: Streptococcus suis]] | ||
| + | [[Category: dTDP-glucose 4,6-dehydratase]] | ||
[[Category: Beis, K.]] | [[Category: Beis, K.]] | ||
[[Category: Naismith, J.H.]] | [[Category: Naismith, J.H.]] | ||
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[[Category: rhamnose]] | [[Category: rhamnose]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:33:52 2007'' |
Revision as of 12:29, 30 October 2007
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THE STRUCTURE OF NADH IN THE DTDP-D-GLUCOSE DEHYDRATASE (RMLB) ENZYME
Overview
The structure of Streptococcus suis serotype type 2 dTDP-d-glucose, 4,6-dehydratase (RmlB) has been determined to 1.5 A resolution with its, nicotinamide coenzyme and substrate analogue dTDP-xylose bound in an, abortive complex. During enzyme turnover, NAD(+) abstracts a hydride from, the C4' atom of dTDP-glucose-forming NADH. After elimination of water, hydride is then transferred back to the C6' atom of, dTDP-4-keto-5,6-glucosene-regenerating NAD(+). Single-crystal, spectroscopic studies unambiguously show that the coenzyme has been, trapped as NADH in the crystal. Electron density clearly demonstrates that, in contrast to native structures of RmlB where a flat nicotinamide ring is, observed, the dihydropyridine ring of the reduced cofactor in this complex, is found as a boat. The si ... [(full description)]
About this Structure
1OC2 is a [Single protein] structure of sequence from [Streptococcus suis] with SO4, TDX and NAD as [ligands]. Active as [dTDP-glucose 4,6-dehydratase], with EC number [4.2.1.46]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The structure of NADH in the enzyme dTDP-d-glucose dehydratase (RmlB)., Beis K, Allard ST, Hegeman AD, Murshudov G, Philp D, Naismith JH, J Am Chem Soc. 2003 Oct 1;125(39):11872-8. PMID:14505409
Page seeded by OCA on Tue Oct 30 14:33:52 2007
Categories: Single protein | Streptococcus suis | DTDP-glucose 4,6-dehydratase | Beis, K. | Naismith, J.H. | NAD | SO4 | TDX | Dehydratase | Lyase | Nadh | Rhamnose
