1kwp
From Proteopedia
(New page: 200px<br /> <applet load="1kwp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kwp, resolution 2.8Å" /> '''Crystal Structure of...) |
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| - | [[Image:1kwp.gif|left|200px]]<br /> | + | [[Image:1kwp.gif|left|200px]]<br /><applet load="1kwp" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1kwp" size=" | + | |
caption="1kwp, resolution 2.8Å" /> | caption="1kwp, resolution 2.8Å" /> | ||
'''Crystal Structure of MAPKAP2'''<br /> | '''Crystal Structure of MAPKAP2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | MAPK-activated protein kinase 2 (MAPKAPK2), one of several kinases | + | MAPK-activated protein kinase 2 (MAPKAPK2), one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response. The activated MAPKAPK2 phosphorylates its nuclear targets CREB/ATF1, serum response factor, and E2A protein E47 and its cytoplasmic targets HSP25/27, LSP-1, 5-lipoxygenase, glycogen synthase, and tyrosine hydroxylase. The crystal structure of unphosphorylated MAPKAPK2, determined at 2.8 A resolution, includes the kinase domain and the C-terminal regulatory domain. Although the protein is inactive, the kinase domain adopts an active conformation with aspartate 366 mimicking the missing phosphorylated threonine 222 in the activation loop. The C-terminal regulatory domain forms a helix-turn-helix plus a long strand. Phosphorylation of threonine 334, which is located between the kinase domain and the C-terminal regulatory domain, may serve as a switch for MAPKAPK2 nuclear import and export. Phosphorylated MAPKAPK2 masks the nuclear localization signal at its C terminus by binding to p38. It unmasks the nuclear export signal, which is part of the second C-terminal helix packed along the surface of kinase domain C-lobe, and thereby carries p38 to the cytoplasm. |
==About this Structure== | ==About this Structure== | ||
| - | 1KWP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1KWP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=HG:'>HG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KWP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Behrens, A | + | [[Category: Behrens, A E.]] |
| - | [[Category: Fitzgibbon, M | + | [[Category: Fitzgibbon, M J.]] |
| - | [[Category: Fulghum, J | + | [[Category: Fulghum, J R.]] |
| - | [[Category: Haar, E | + | [[Category: Haar, E ter.]] |
[[Category: Hayakawa, K.]] | [[Category: Hayakawa, K.]] | ||
| - | [[Category: Lippke, J | + | [[Category: Lippke, J A.]] |
[[Category: Meng, W.]] | [[Category: Meng, W.]] | ||
| - | [[Category: Swenson, L | + | [[Category: Swenson, L L.]] |
[[Category: HG]] | [[Category: HG]] | ||
[[Category: crystallography]] | [[Category: crystallography]] | ||
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[[Category: signal transduction]] | [[Category: signal transduction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:38:45 2008'' |
Revision as of 11:38, 21 February 2008
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Crystal Structure of MAPKAP2
Overview
MAPK-activated protein kinase 2 (MAPKAPK2), one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response. The activated MAPKAPK2 phosphorylates its nuclear targets CREB/ATF1, serum response factor, and E2A protein E47 and its cytoplasmic targets HSP25/27, LSP-1, 5-lipoxygenase, glycogen synthase, and tyrosine hydroxylase. The crystal structure of unphosphorylated MAPKAPK2, determined at 2.8 A resolution, includes the kinase domain and the C-terminal regulatory domain. Although the protein is inactive, the kinase domain adopts an active conformation with aspartate 366 mimicking the missing phosphorylated threonine 222 in the activation loop. The C-terminal regulatory domain forms a helix-turn-helix plus a long strand. Phosphorylation of threonine 334, which is located between the kinase domain and the C-terminal regulatory domain, may serve as a switch for MAPKAPK2 nuclear import and export. Phosphorylated MAPKAPK2 masks the nuclear localization signal at its C terminus by binding to p38. It unmasks the nuclear export signal, which is part of the second C-terminal helix packed along the surface of kinase domain C-lobe, and thereby carries p38 to the cytoplasm.
About this Structure
1KWP is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of mitogen-activated protein kinase-activated protein (MAPKAP) kinase 2 suggests a bifunctional switch that couples kinase activation with nuclear export., Meng W, Swenson LL, Fitzgibbon MJ, Hayakawa K, Ter Haar E, Behrens AE, Fulghum JR, Lippke JA, J Biol Chem. 2002 Oct 4;277(40):37401-5. Epub 2002 Aug 8. PMID:12171911
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