1l1v
From Proteopedia
(New page: 200px<br /><applet load="1l1v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l1v" /> '''UNUSUAL ACTD/DNA_TA COMPLEX STRUCTURE'''<br ...) |
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| - | [[Image:1l1v.gif|left|200px]]<br /><applet load="1l1v" size=" | + | [[Image:1l1v.gif|left|200px]]<br /><applet load="1l1v" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l1v" /> | caption="1l1v" /> | ||
'''UNUSUAL ACTD/DNA_TA COMPLEX STRUCTURE'''<br /> | '''UNUSUAL ACTD/DNA_TA COMPLEX STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Many anticancer drugs interact directly with DNA to exert their biological | + | Many anticancer drugs interact directly with DNA to exert their biological functions. To date, all noncovalent, intercalating drugs interact with DNA exclusively by inserting their chromophores into base steps to form elongated and unwound duplex structures without disrupting the flanking base pairs. By using actinomycin D (ActD)-5'-GXC/CYG-5' complexes as examples, we have found a rather unusual interaction mode for the intercalated drug; the central Watson-Crick X/Y base pairs are looped out and displaced by the ActD chromophore. The looped-out bases are not disordered but interact perpendicularly with the base/chromophore and form specific H bonds with DNA. Such a complex structure provides intriguing insights into how ligand interacts with DNA and enlarges the repertoires for sequence-specific DNA recognition. |
==About this Structure== | ==About this Structure== | ||
| - | 1L1V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1L1V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1V OCA]. |
==Reference== | ==Reference== | ||
Looped out and perpendicular: deformation of Watson-Crick base pair associated with actinomycin D binding., Chou SH, Chin KH, Chen FM, Proc Natl Acad Sci U S A. 2002 May 14;99(10):6625-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12011426 12011426] | Looped out and perpendicular: deformation of Watson-Crick base pair associated with actinomycin D binding., Chou SH, Chin KH, Chen FM, Proc Natl Acad Sci U S A. 2002 May 14;99(10):6625-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12011426 12011426] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Chen, F | + | [[Category: Chen, F M.]] |
| - | [[Category: Chin, K | + | [[Category: Chin, K H.]] |
| - | [[Category: Chou, S | + | [[Category: Chou, S H.]] |
[[Category: actd/dna complex]] | [[Category: actd/dna complex]] | ||
[[Category: looped-out]] | [[Category: looped-out]] | ||
[[Category: perpendicular]] | [[Category: perpendicular]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:20 2008'' |
Revision as of 11:40, 21 February 2008
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UNUSUAL ACTD/DNA_TA COMPLEX STRUCTURE
Overview
Many anticancer drugs interact directly with DNA to exert their biological functions. To date, all noncovalent, intercalating drugs interact with DNA exclusively by inserting their chromophores into base steps to form elongated and unwound duplex structures without disrupting the flanking base pairs. By using actinomycin D (ActD)-5'-GXC/CYG-5' complexes as examples, we have found a rather unusual interaction mode for the intercalated drug; the central Watson-Crick X/Y base pairs are looped out and displaced by the ActD chromophore. The looped-out bases are not disordered but interact perpendicularly with the base/chromophore and form specific H bonds with DNA. Such a complex structure provides intriguing insights into how ligand interacts with DNA and enlarges the repertoires for sequence-specific DNA recognition.
About this Structure
1L1V is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Looped out and perpendicular: deformation of Watson-Crick base pair associated with actinomycin D binding., Chou SH, Chin KH, Chen FM, Proc Natl Acad Sci U S A. 2002 May 14;99(10):6625-30. PMID:12011426
Page seeded by OCA on Thu Feb 21 13:40:20 2008
