1l2h
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The crystal structure at 1.54 A resolution of a double mutant of | + | The crystal structure at 1.54 A resolution of a double mutant of interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was determined by multiple-wavelength anomalous dispersion (MAD) using data from highly twinned selenomethionine-modified crystals. The space group is P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation function analysis and various intensity statistics revealed the presence of merohedral twinning in crystals of both the native (twinning fraction alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms. Structure determination and refinement are discussed with emphasis on the possible reasons for successful phasing using untreated twinned MAD data. |
==Disease== | ==Disease== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Heidary, D | + | [[Category: Heidary, D K.]] |
| - | [[Category: Jennings, P | + | [[Category: Jennings, P A.]] |
| - | [[Category: Kelker, M | + | [[Category: Kelker, M S.]] |
[[Category: Oseroff, V.]] | [[Category: Oseroff, V.]] | ||
| - | [[Category: Rudolph, M | + | [[Category: Rudolph, M G.]] |
| - | [[Category: Schneider, T | + | [[Category: Schneider, T R.]] |
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A.]] |
| - | [[Category: Yeates, T | + | [[Category: Yeates, T O.]] |
[[Category: beta-trefoil]] | [[Category: beta-trefoil]] | ||
[[Category: f42w/w120f double mutant]] | [[Category: f42w/w120f double mutant]] | ||
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[[Category: protein folding]] | [[Category: protein folding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:34 2008'' |
Revision as of 11:40, 21 February 2008
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Crystal structure of Interleukin 1-beta F42W/W120F mutant
Contents |
Overview
The crystal structure at 1.54 A resolution of a double mutant of interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was determined by multiple-wavelength anomalous dispersion (MAD) using data from highly twinned selenomethionine-modified crystals. The space group is P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation function analysis and various intensity statistics revealed the presence of merohedral twinning in crystals of both the native (twinning fraction alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms. Structure determination and refinement are discussed with emphasis on the possible reasons for successful phasing using untreated twinned MAD data.
Disease
Known disease associated with this structure: Gastric cancer risk after H. pylori infection OMIM:[147720]
About this Structure
1L2H is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1beta., Rudolph MG, Kelker MS, Schneider TR, Yeates TO, Oseroff V, Heidary DK, Jennings PA, Wilson IA, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):290-8. Epub 2003, Jan 23. PMID:12554939
Page seeded by OCA on Thu Feb 21 13:40:34 2008
