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1l2k
From Proteopedia
(New page: 200px<br /><applet load="1l2k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l2k, resolution 1.50Å" /> '''Neutron Structure De...) |
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| - | [[Image:1l2k.gif|left|200px]]<br /><applet load="1l2k" size=" | + | [[Image:1l2k.gif|left|200px]]<br /><applet load="1l2k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l2k, resolution 1.50Å" /> | caption="1l2k, resolution 1.50Å" /> | ||
'''Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.'''<br /> | '''Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | From the first days of protein neutron structure determination sperm whale | + | From the first days of protein neutron structure determination sperm whale myoglobin was an object under investigation [Nature 224 (1969) 143, J. Mol. Biol. 220 (1991) 381]. Nevertheless myoglobin is still of interest [Proc. Natl. Acad. Sci. USA 97 (2000) 3872]. The feasibility of the monochromatic neutron diffractometer BIX-3 at the JRR-3M reactor at the JAERI [J. Phys. Chem. Solids 60 (1999) 1623], to collect high-resolution diffraction data in a relatively short time stimulated us to repeat the structural determination of myoglobin. The structure of metmyoglobin has been determined up to a resolution of 1.5 A. The hydrogen atoms were replaced in part, by deuterium soaking the crystals for more than 10 years in D(2)O. A refinement of all atoms has been performed including the refinement of individual mean square displacements and occupancies of the exchangeable protons in backbone hydrogen bonds. A method is described to show clear negative scattering densities of the H atoms. Water molecules within the protein and on the molecule surface are shown. The exchangeability of H atoms is correlated with structural distribution and flexibility. |
==About this Structure== | ==About this Structure== | ||
| - | 1L2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with SO4, ND4, HEM and DOD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1L2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=ND4:'>ND4</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=DOD:'>DOD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Niimura, N.]] | [[Category: Niimura, N.]] | ||
[[Category: Ostermann, A.]] | [[Category: Ostermann, A.]] | ||
| - | [[Category: Parak, F | + | [[Category: Parak, F G.]] |
[[Category: Tanaka, I.]] | [[Category: Tanaka, I.]] | ||
[[Category: DOD]] | [[Category: DOD]] | ||
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[[Category: neutron structure]] | [[Category: neutron structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:35 2008'' |
Revision as of 11:40, 21 February 2008
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Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.
Overview
From the first days of protein neutron structure determination sperm whale myoglobin was an object under investigation [Nature 224 (1969) 143, J. Mol. Biol. 220 (1991) 381]. Nevertheless myoglobin is still of interest [Proc. Natl. Acad. Sci. USA 97 (2000) 3872]. The feasibility of the monochromatic neutron diffractometer BIX-3 at the JRR-3M reactor at the JAERI [J. Phys. Chem. Solids 60 (1999) 1623], to collect high-resolution diffraction data in a relatively short time stimulated us to repeat the structural determination of myoglobin. The structure of metmyoglobin has been determined up to a resolution of 1.5 A. The hydrogen atoms were replaced in part, by deuterium soaking the crystals for more than 10 years in D(2)O. A refinement of all atoms has been performed including the refinement of individual mean square displacements and occupancies of the exchangeable protons in backbone hydrogen bonds. A method is described to show clear negative scattering densities of the H atoms. Water molecules within the protein and on the molecule surface are shown. The exchangeability of H atoms is correlated with structural distribution and flexibility.
About this Structure
1L2K is a Single protein structure of sequence from Physeter catodon with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:12062378
Page seeded by OCA on Thu Feb 21 13:40:35 2008
Categories: Physeter catodon | Single protein | Engler, N. | Niimura, N. | Ostermann, A. | Parak, F G. | Tanaka, I. | DOD | HEM | ND4 | SO4 | Heme protein | Hydration structure | Hydrogen atoms | Neutron structure
