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1l3z
From Proteopedia
(New page: 200px<br /><applet load="1l3z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l3z, resolution 2.01Å" /> '''Crystal Structure An...) |
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| - | [[Image:1l3z.gif|left|200px]]<br /><applet load="1l3z" size=" | + | [[Image:1l3z.gif|left|200px]]<br /><applet load="1l3z" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l3z, resolution 2.01Å" /> | caption="1l3z, resolution 2.01Å" /> | ||
'''Crystal Structure Analysis of an RNA Heptamer'''<br /> | '''Crystal Structure Analysis of an RNA Heptamer'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of an alternating RNA heptamer r(GUAUACA) has been | + | The crystal structure of an alternating RNA heptamer r(GUAUACA) has been determined to 2.0 A resolution and refined to an R(work) of 17.1% and R(free) of 18.5% using 2797 reflections. The heptamer crystallized in the space group C222 with a unit cell of a = 25.74, b = 106.58, c = 30.26 A and two independent strands in the asymmetric unit. Each heptamer forms a duplex with its symmetry-related strand and each duplex contains six Watson-Crick base pairs and 3'-end adenosine overhangs. Therefore, two kinds of duplex (duplex 1 and duplex 2) are formed. Duplexes 1 stack on each other forming a pseudo-continuous column, which is typical of the RNA packing mode, while duplex 2 is typical of A-DNA packing with its termini in abutting interactions. Overhang adenine residues stack within the duplexes with C3'-endo sugar pucker and C2'-endo sugar pucker in duplexes 1 and 2, respectively. A Na+ ion in the crystal lattice is water bridged to two N1 atoms of symmetry-related A7 bases. |
==About this Structure== | ==About this Structure== | ||
| - | 1L3Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1L3Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3Z OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rna]] | [[Category: rna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:41:04 2008'' |
Revision as of 11:41, 21 February 2008
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Crystal Structure Analysis of an RNA Heptamer
Overview
The crystal structure of an alternating RNA heptamer r(GUAUACA) has been determined to 2.0 A resolution and refined to an R(work) of 17.1% and R(free) of 18.5% using 2797 reflections. The heptamer crystallized in the space group C222 with a unit cell of a = 25.74, b = 106.58, c = 30.26 A and two independent strands in the asymmetric unit. Each heptamer forms a duplex with its symmetry-related strand and each duplex contains six Watson-Crick base pairs and 3'-end adenosine overhangs. Therefore, two kinds of duplex (duplex 1 and duplex 2) are formed. Duplexes 1 stack on each other forming a pseudo-continuous column, which is typical of the RNA packing mode, while duplex 2 is typical of A-DNA packing with its termini in abutting interactions. Overhang adenine residues stack within the duplexes with C3'-endo sugar pucker and C2'-endo sugar pucker in duplexes 1 and 2, respectively. A Na+ ion in the crystal lattice is water bridged to two N1 atoms of symmetry-related A7 bases.
About this Structure
1L3Z is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of an alternating RNA heptamer r(GUAUACA) forming a six base-paired duplex with 3'-end adenine overhangs., Shi K, Pan B, Sundaralingam M, Nucleic Acids Res. 2003 Mar 1;31(5):1392-7. PMID:12595546
Page seeded by OCA on Thu Feb 21 13:41:04 2008
Categories: Protein complex | Pan, B. | Shi, K. | Sundaralingam, M. | NA | Rna
