1lgl
From Proteopedia
(New page: 200px<br /><applet load="1lgl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lgl" /> '''Solution structure of HERG-specific scorpion...) |
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| - | [[Image:1lgl.gif|left|200px]]<br /><applet load="1lgl" size=" | + | [[Image:1lgl.gif|left|200px]]<br /><applet load="1lgl" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution structure of HERG-specific scorpion toxin BeKm-1'''<br /> | '''Solution structure of HERG-specific scorpion toxin BeKm-1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The scorpion toxin BeKm-1 is unique among a variety of known short | + | The scorpion toxin BeKm-1 is unique among a variety of known short scorpion toxins affecting potassium channels in its selective action on ether-a-go-go-related gene (ERG)-type channels. BeKm-1 shares the common molecular scaffold with other short scorpion toxins. The toxin spatial structure resolved by NMR consists of a short alpha-helix and a triple-stranded antiparallel beta-sheet. By toxin mutagenesis study we identified the residues that are important for the binding of BeKm-1 to the human ERG K+ (HERG) channel. The most critical residues (Tyr-11, Lys-18, Arg-20, Lys-23) are located in the alpha-helix and following loop whereas the "traditional" functional site of other short scorpion toxins is formed by residues from the beta-sheet. Thus the unique location of the binding site of BeKm-1 provides its specificity toward the HERG channel. |
==About this Structure== | ==About this Structure== | ||
| - | 1LGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_eupeus Mesobuthus eupeus]. Full crystallographic information is available from [http:// | + | 1LGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_eupeus Mesobuthus eupeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LGL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Angelo, K.]] | [[Category: Angelo, K.]] | ||
| - | [[Category: Arseniev, A | + | [[Category: Arseniev, A S.]] |
| - | [[Category: Bocharov, E | + | [[Category: Bocharov, E V.]] |
| - | [[Category: Grinenko, O | + | [[Category: Grinenko, O V.]] |
| - | [[Category: Grishin, E | + | [[Category: Grishin, E V.]] |
| - | [[Category: Korolokova, Y | + | [[Category: Korolokova, Y V.]] |
| - | [[Category: Lipkin, A | + | [[Category: Lipkin, A V.]] |
| - | [[Category: Maslennikov, I | + | [[Category: Maslennikov, I V.]] |
| - | [[Category: Nosireva, E | + | [[Category: Nosireva, E D.]] |
| - | [[Category: Olesen, S | + | [[Category: Olesen, S P.]] |
| - | [[Category: Pluzhnikov, K | + | [[Category: Pluzhnikov, K A.]] |
[[Category: alpha-beta motif]] | [[Category: alpha-beta motif]] | ||
[[Category: cysteine-knot motif]] | [[Category: cysteine-knot motif]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:44:43 2008'' |
Revision as of 11:44, 21 February 2008
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Solution structure of HERG-specific scorpion toxin BeKm-1
Overview
The scorpion toxin BeKm-1 is unique among a variety of known short scorpion toxins affecting potassium channels in its selective action on ether-a-go-go-related gene (ERG)-type channels. BeKm-1 shares the common molecular scaffold with other short scorpion toxins. The toxin spatial structure resolved by NMR consists of a short alpha-helix and a triple-stranded antiparallel beta-sheet. By toxin mutagenesis study we identified the residues that are important for the binding of BeKm-1 to the human ERG K+ (HERG) channel. The most critical residues (Tyr-11, Lys-18, Arg-20, Lys-23) are located in the alpha-helix and following loop whereas the "traditional" functional site of other short scorpion toxins is formed by residues from the beta-sheet. Thus the unique location of the binding site of BeKm-1 provides its specificity toward the HERG channel.
About this Structure
1LGL is a Single protein structure of sequence from Mesobuthus eupeus. Full crystallographic information is available from OCA.
Reference
New binding site on common molecular scaffold provides HERG channel specificity of scorpion toxin BeKm-1., Korolkova YV, Bocharov EV, Angelo K, Maslennikov IV, Grinenko OV, Lipkin AV, Nosyreva ED, Pluzhnikov KA, Olesen SP, Arseniev AS, Grishin EV, J Biol Chem. 2002 Nov 8;277(45):43104-9. Epub 2002 Jul 31. PMID:12151390
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