2c5s

From Proteopedia

Revision as of 12:32, 30 October 2007

CRYSTAL STRUCTURE OF BACILLUS ANTHRACIS THII, A TRNA-MODIFYING ENZYME CONTAINING THE PREDICTED RNA-BINDING THUMP DOMAIN

Overview

ThiI is an enzyme responsible for the formation of the modified base S(4)U, (4-thiouridine) found at position 8 in some prokaryotic tRNAs. This base, acts as a sensitive trigger for the response mechanism to UV exposure, providing protection against its damaging effects. We present the crystal, structure of Bacillus anthracis ThiI in complex with AMP, revealing an, extended tripartite architecture in which an N-terminal ferredoxin-like, domain (NFLD) connects the C-terminal catalytic PP-loop pyrophosphatase, domain with a THUMP domain, an ancient predicted RNA-binding domain that, is widespread in all kingdoms of life. We describe the structure of the, THUMP domain, which appears to be unrelated to RNA-binding domains of, known structure. Mapping the conserved residues of NFLD and the ... [(full description)]

About this Structure

2C5S is a [Single protein] structure of sequence from [Bacillus anthracis] with AMP as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain., Waterman DG, Ortiz-Lombardia M, Fogg MJ, Koonin EV, Antson AA, J Mol Biol. 2006 Feb 10;356(1):97-110. Epub 2005 Nov 22. PMID:16343540

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