This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1w4p
From Proteopedia
| Line 29: | Line 29: | ||
[[Category: rnase a]] | [[Category: rnase a]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:38:36 2007'' |
Revision as of 12:33, 30 October 2007
|
BINDING OF NONNATURAL 3'-NUCLEOTIDES TO RIBONUCLEASE A
Overview
2'-Fluoro-2'-deoxyuridine 3'-phosphate (dU(F)MP) and arabinouridine, 3'-phosphate (araUMP) have non-natural furanose rings. dU(F)MP and araUMP, were prepared by chemical synthesis and found to have three- to sevenfold, higher affinity than uridine 3'-phosphate (3'-UMP) or 2'-deoxyuridine, 3'-phosphate (dUMP) for ribonuclease A (RNase A). These differences, probably arise (in part) from the phosphoryl groups of 3'-UMP, dU(F)MP, and araUMP (pK(a) = 5.9) being more anionic than that of dUMP (pK(a) =, 6.3). The three-dimensional structures of the crystalline complexes of, RNase A with dUMP, dU(F)MP and araUMP were determined at < 1.7 A, resolution by X-ray diffraction analysis. In these three structures, the, uracil nucleobases and phosphoryl groups bind to the enzyme in a nearly, ... [(full description)]
About this Structure
1W4P is a [Single protein] structure of sequence from [Bos taurus] with UM3 as [ligand]. Active as [Pancreatic ribonuclease], with EC number [3.1.27.5]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Binding of non-natural 3'-nucleotides to ribonuclease A., Jenkins CL, Thiyagarajan N, Sweeney RY, Guy MP, Kelemen BR, Acharya KR, Raines RT, FEBS J. 2005 Feb;272(3):744-55. PMID:15670155
Page seeded by OCA on Tue Oct 30 14:38:36 2007
