This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1lwv
From Proteopedia
(New page: 200px<br /> <applet load="1lwv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lwv, resolution 2.3Å" /> '''Borohydride-trapped ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1lwv.gif|left|200px]]<br /> | + | [[Image:1lwv.gif|left|200px]]<br /><applet load="1lwv" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1lwv" size=" | + | |
caption="1lwv, resolution 2.3Å" /> | caption="1lwv, resolution 2.3Å" /> | ||
'''Borohydride-trapped hOgg1 Intermediate Structure Co-Crystallized with 8-aminoguanine'''<br /> | '''Borohydride-trapped hOgg1 Intermediate Structure Co-Crystallized with 8-aminoguanine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Most spontaneous damage to bases in DNA is corrected through the action of | + | Most spontaneous damage to bases in DNA is corrected through the action of the base-excision DNA repair pathway. Base excision repair is initiated by DNA glycosylases, lesion-specific enzymes that intercept aberrant bases in DNA and catalyze their excision. How such proteins accomplish the feat of catalyzing no fewer than five sequential reaction steps using a single active site has been unknown. To help answer this, we report the structure of a trapped catalytic intermediate in DNA repair by human 8-oxoguanine DNA glycosylase. This structure and supporting biochemical results reveal that the enzyme sequesters the excised lesion base and exploits it as a cofactor to participate in catalysis. To our knowledge, the present example represents the first documented case of product-assisted catalysis in an enzyme-catalyzed reaction. |
==Disease== | ==Disease== | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1LWV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and ANG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1LWV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ANG:'>ANG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWV OCA]. |
==Reference== | ==Reference== | ||
| Line 17: | Line 16: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bruner, S | + | [[Category: Bruner, S D.]] |
| - | [[Category: Fromme, J | + | [[Category: Fromme, J C.]] |
[[Category: Karplus, M.]] | [[Category: Karplus, M.]] | ||
| - | [[Category: Verdine, G | + | [[Category: Verdine, G L.]] |
[[Category: Yang, W.]] | [[Category: Yang, W.]] | ||
[[Category: ANG]] | [[Category: ANG]] | ||
| Line 32: | Line 31: | ||
[[Category: reaction intermediate]] | [[Category: reaction intermediate]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:17 2008'' |
Revision as of 11:49, 21 February 2008
|
Borohydride-trapped hOgg1 Intermediate Structure Co-Crystallized with 8-aminoguanine
Contents |
Overview
Most spontaneous damage to bases in DNA is corrected through the action of the base-excision DNA repair pathway. Base excision repair is initiated by DNA glycosylases, lesion-specific enzymes that intercept aberrant bases in DNA and catalyze their excision. How such proteins accomplish the feat of catalyzing no fewer than five sequential reaction steps using a single active site has been unknown. To help answer this, we report the structure of a trapped catalytic intermediate in DNA repair by human 8-oxoguanine DNA glycosylase. This structure and supporting biochemical results reveal that the enzyme sequesters the excised lesion base and exploits it as a cofactor to participate in catalysis. To our knowledge, the present example represents the first documented case of product-assisted catalysis in an enzyme-catalyzed reaction.
Disease
Known disease associated with this structure: Renal cell carcinoma, clear cell, somatic OMIM:[601982]
About this Structure
1LWV is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Product-assisted catalysis in base-excision DNA repair., Fromme JC, Bruner SD, Yang W, Karplus M, Verdine GL, Nat Struct Biol. 2003 Mar;10(3):204-11. PMID:12592398
Page seeded by OCA on Thu Feb 21 13:49:17 2008
