1m6u
From Proteopedia
(New page: 200px<br /><applet load="1m6u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m6u, resolution 2.3Å" /> '''Crystal Structure of ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1m6u.jpg|left|200px]]<br /><applet load="1m6u" size=" | + | [[Image:1m6u.jpg|left|200px]]<br /><applet load="1m6u" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1m6u, resolution 2.3Å" /> | caption="1m6u, resolution 2.3Å" /> | ||
'''Crystal Structure of a Novel DNA-binding domain from Ndt80, a Transcriptional Activator Required for Meiosis in Yeast'''<br /> | '''Crystal Structure of a Novel DNA-binding domain from Ndt80, a Transcriptional Activator Required for Meiosis in Yeast'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ndt80 is a transcriptional activator required for meiosis in the yeast | + | Ndt80 is a transcriptional activator required for meiosis in the yeast Saccharomyces cerevisiae. Here, we report the crystal structure at 2.3 A resolution of the DNA-binding domain of Ndt80 experimentally phased by using the anomalous and isomorphous signal from a single ordered Se atom per molecule of 272-aa residues. The structure reveals a single approximately 32-kDa domain with a distinct fold comprising a beta-sandwich core elaborated with seven additional beta-sheets and three short alpha-helices. Inspired by the structure, we have performed a mutational analysis and defined a DNA-binding motif in this domain. The DNA-binding domain of Ndt80 is homologous to a number of proteins from higher eukaryotes, and the residues that we have shown are required for DNA binding by Ndt80 are highly conserved among this group of proteins. These results suggest that Ndt80 is the defining member of a previously uncharacterized family of transcription factors, including the human protein (C11orf9), which has been shown to be highly expressed in invasive or metastatic tumor cells. |
==About this Structure== | ==About this Structure== | ||
| - | 1M6U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1M6U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M6U OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cote, M | + | [[Category: Cote, M L.]] |
[[Category: Fingerman, I.]] | [[Category: Fingerman, I.]] | ||
| - | [[Category: Georgiadis, M | + | [[Category: Georgiadis, M M.]] |
| - | [[Category: Montano, S | + | [[Category: Montano, S P.]] |
[[Category: Pierce, M.]] | [[Category: Pierce, M.]] | ||
| - | [[Category: Vershon, A | + | [[Category: Vershon, A K.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: dna-binding]] | [[Category: dna-binding]] | ||
| Line 25: | Line 25: | ||
[[Category: yeast protein]] | [[Category: yeast protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:52:07 2008'' |
Revision as of 11:52, 21 February 2008
|
Crystal Structure of a Novel DNA-binding domain from Ndt80, a Transcriptional Activator Required for Meiosis in Yeast
Overview
Ndt80 is a transcriptional activator required for meiosis in the yeast Saccharomyces cerevisiae. Here, we report the crystal structure at 2.3 A resolution of the DNA-binding domain of Ndt80 experimentally phased by using the anomalous and isomorphous signal from a single ordered Se atom per molecule of 272-aa residues. The structure reveals a single approximately 32-kDa domain with a distinct fold comprising a beta-sandwich core elaborated with seven additional beta-sheets and three short alpha-helices. Inspired by the structure, we have performed a mutational analysis and defined a DNA-binding motif in this domain. The DNA-binding domain of Ndt80 is homologous to a number of proteins from higher eukaryotes, and the residues that we have shown are required for DNA binding by Ndt80 are highly conserved among this group of proteins. These results suggest that Ndt80 is the defining member of a previously uncharacterized family of transcription factors, including the human protein (C11orf9), which has been shown to be highly expressed in invasive or metastatic tumor cells.
About this Structure
1M6U is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast., Montano SP, Cote ML, Fingerman I, Pierce M, Vershon AK, Georgiadis MM, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14041-6. Epub 2002 Oct 16. PMID:12384578
Page seeded by OCA on Thu Feb 21 13:52:07 2008
