1mby
From Proteopedia
(New page: 200px<br /><applet load="1mby" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mby, resolution 2.0Å" /> '''Murine Sak Polo Domai...) |
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| - | [[Image:1mby.jpg|left|200px]]<br /><applet load="1mby" size=" | + | [[Image:1mby.jpg|left|200px]]<br /><applet load="1mby" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mby, resolution 2.0Å" /> | caption="1mby, resolution 2.0Å" /> | ||
'''Murine Sak Polo Domain'''<br /> | '''Murine Sak Polo Domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The small family of polo-like kinases (Plks) includes Cdc5 from | + | The small family of polo-like kinases (Plks) includes Cdc5 from Saccharomyces cerevisiae, Plo1 from Schizosaccharomyces pombe, Polo from Drosophila melanogaster and the four mammalian genes Plk1, Prk/Fnk, Snk and Sak. These kinases control cell cycle progression through the regulation of centrosome maturation and separation, mitotic entry, metaphase to anaphase transition, mitotic exit and cytokinesis. Plks are characterized by an N-terminal Ser/Thr protein kinase domain and the presence of one or two C-terminal regions of similarity, termed the polo box motifs. These motifs have been demonstrated for Cdc5 and Plk1 to be required for mitotic progression and for subcellular localization to mitotic structures. Here we report the 2.0 A crystal structure of a novel domain composed of the polo box motif of murine Sak. The structure consists of a dimeric fold with a deep interfacial cleft and pocket, suggestive of a ligand-binding site. We show that this domain forms homodimers both in vitro and in vivo, and localizes to centrosomes and the cleavage furrow during cytokinesis. The requirement of the polo domain for Plk family function and the unique physical properties of the domain identify it as an attractive target for inhibitor design. |
==About this Structure== | ==About this Structure== | ||
| - | 1MBY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http:// | + | 1MBY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MBY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Davidson, A.]] | [[Category: Davidson, A.]] | ||
| - | [[Category: Dennis, J | + | [[Category: Dennis, J W.]] |
| - | [[Category: Hudson, J | + | [[Category: Hudson, J W.]] |
[[Category: Kozarova, A.]] | [[Category: Kozarova, A.]] | ||
| - | [[Category: Leung, G | + | [[Category: Leung, G C.]] |
[[Category: Sicheri, F.]] | [[Category: Sicheri, F.]] | ||
[[Category: polo domain]] | [[Category: polo domain]] | ||
[[Category: strand exchange]] | [[Category: strand exchange]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:53:40 2008'' |
Revision as of 11:53, 21 February 2008
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Murine Sak Polo Domain
Overview
The small family of polo-like kinases (Plks) includes Cdc5 from Saccharomyces cerevisiae, Plo1 from Schizosaccharomyces pombe, Polo from Drosophila melanogaster and the four mammalian genes Plk1, Prk/Fnk, Snk and Sak. These kinases control cell cycle progression through the regulation of centrosome maturation and separation, mitotic entry, metaphase to anaphase transition, mitotic exit and cytokinesis. Plks are characterized by an N-terminal Ser/Thr protein kinase domain and the presence of one or two C-terminal regions of similarity, termed the polo box motifs. These motifs have been demonstrated for Cdc5 and Plk1 to be required for mitotic progression and for subcellular localization to mitotic structures. Here we report the 2.0 A crystal structure of a novel domain composed of the polo box motif of murine Sak. The structure consists of a dimeric fold with a deep interfacial cleft and pocket, suggestive of a ligand-binding site. We show that this domain forms homodimers both in vitro and in vivo, and localizes to centrosomes and the cleavage furrow during cytokinesis. The requirement of the polo domain for Plk family function and the unique physical properties of the domain identify it as an attractive target for inhibitor design.
About this Structure
1MBY is a Single protein structure of sequence from Mus musculus. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
Reference
The Sak polo-box comprises a structural domain sufficient for mitotic subcellular localization., Leung GC, Hudson JW, Kozarova A, Davidson A, Dennis JW, Sicheri F, Nat Struct Biol. 2002 Oct;9(10):719-24. PMID:12352953
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