1n11
From Proteopedia
(New page: 200px<br /> <applet load="1n11" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n11, resolution 2.70Å" /> '''D34 REGION OF HUMAN...) |
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| - | [[Image:1n11.gif|left|200px]]<br /> | + | [[Image:1n11.gif|left|200px]]<br /><applet load="1n11" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1n11" size=" | + | |
caption="1n11, resolution 2.70Å" /> | caption="1n11, resolution 2.70Å" /> | ||
'''D34 REGION OF HUMAN ANKYRIN-R AND LINKER'''<br /> | '''D34 REGION OF HUMAN ANKYRIN-R AND LINKER'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ankyrins are multifunctional adaptors that link specific proteins to the | + | Ankyrins are multifunctional adaptors that link specific proteins to the membrane-associated, spectrin- actin cytoskeleton. The N-terminal, 'membrane-binding' domain of ankyrins contains 24 ANK repeats and mediates most binding activities. Repeats 13-24 are especially active, with known sites of interaction for the Na/K ATPase, Cl/HCO(3) anion exchanger, voltage-gated sodium channel, clathrin heavy chain and L1 family cell adhesion molecules. Here we report the crystal structure of a human ankyrinR construct containing ANK repeats 13-24 and a portion of the spectrin-binding domain. The ANK repeats are observed to form a contiguous spiral stack with which the spectrin-binding domain fragment associates as an extended strand. The structural information has been used to construct models of all 24 repeats of the membrane-binding domain as well as the interactions of the repeats with the Cl/HCO(3) anion exchanger and clathrin. These models, together with available binding studies, suggest that ion transporters such as the anion exchanger associate in a large central cavity formed by the ANK repeat spiral, while clathrin and cell adhesion molecules associate with specific regions outside this cavity. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1N11 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with BR and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1N11 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=BR:'>BR</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N11 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Anderson, R | + | [[Category: Anderson, R G.W.]] |
[[Category: Machius, M.]] | [[Category: Machius, M.]] | ||
[[Category: Michaely, P.]] | [[Category: Michaely, P.]] | ||
| - | [[Category: Tomchick, D | + | [[Category: Tomchick, D R.]] |
[[Category: BR]] | [[Category: BR]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: clathrin]] | [[Category: clathrin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:05 2008'' |
Revision as of 12:01, 21 February 2008
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D34 REGION OF HUMAN ANKYRIN-R AND LINKER
Contents |
Overview
Ankyrins are multifunctional adaptors that link specific proteins to the membrane-associated, spectrin- actin cytoskeleton. The N-terminal, 'membrane-binding' domain of ankyrins contains 24 ANK repeats and mediates most binding activities. Repeats 13-24 are especially active, with known sites of interaction for the Na/K ATPase, Cl/HCO(3) anion exchanger, voltage-gated sodium channel, clathrin heavy chain and L1 family cell adhesion molecules. Here we report the crystal structure of a human ankyrinR construct containing ANK repeats 13-24 and a portion of the spectrin-binding domain. The ANK repeats are observed to form a contiguous spiral stack with which the spectrin-binding domain fragment associates as an extended strand. The structural information has been used to construct models of all 24 repeats of the membrane-binding domain as well as the interactions of the repeats with the Cl/HCO(3) anion exchanger and clathrin. These models, together with available binding studies, suggest that ion transporters such as the anion exchanger associate in a large central cavity formed by the ANK repeat spiral, while clathrin and cell adhesion molecules associate with specific regions outside this cavity.
Disease
Known diseases associated with this structure: Chondrocalcinosis 2 OMIM:[605145], Craniometaphyseal dysplasia OMIM:[605145]
About this Structure
1N11 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of a 12 ANK repeat stack from human ankyrinR., Michaely P, Tomchick DR, Machius M, Anderson RG, EMBO J. 2002 Dec 2;21(23):6387-96. PMID:12456646
Page seeded by OCA on Thu Feb 21 14:01:05 2008
Categories: Homo sapiens | Single protein | Anderson, R G.W. | Machius, M. | Michaely, P. | Tomchick, D R. | BR | CL | Anion exchanger | Ankyrin | Band 3 | Clathrin
