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1n36
From Proteopedia
(New page: 200px<br /><applet load="1n36" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n36, resolution 3.65Å" /> '''Structure of the The...) |
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| - | [[Image:1n36.gif|left|200px]]<br /><applet load="1n36" size=" | + | [[Image:1n36.gif|left|200px]]<br /><applet load="1n36" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n36, resolution 3.65Å" /> | caption="1n36, resolution 3.65Å" /> | ||
'''Structure of the Thermus thermophilus 30S ribosomal subunit in the presence of crystallographically disordered codon and near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position'''<br /> | '''Structure of the Thermus thermophilus 30S ribosomal subunit in the presence of crystallographically disordered codon and near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A structural and mechanistic explanation for the selection of tRNAs by the | + | A structural and mechanistic explanation for the selection of tRNAs by the ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound at the decoding center and compare affinities of equivalent complexes in solution. In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove. As a result, the transition to a closed form of the 30S induced by cognate tRNA is unfavorable for near-cognate tRNA unless paromomycin induces part of the rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity. |
==About this Structure== | ==About this Structure== | ||
| - | 1N36 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1N36 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N36 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
| - | [[Category: IV, F | + | [[Category: IV, F V.Murphy.]] |
| - | [[Category: Ogle, J | + | [[Category: Ogle, J M.]] |
[[Category: Ramakrishnan, V.]] | [[Category: Ramakrishnan, V.]] | ||
| - | [[Category: Tarry, M | + | [[Category: Tarry, M J.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: 30s ribosomal subunit]] | [[Category: 30s ribosomal subunit]] | ||
| Line 37: | Line 37: | ||
[[Category: wobble]] | [[Category: wobble]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:43 2008'' |
Revision as of 12:01, 21 February 2008
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Structure of the Thermus thermophilus 30S ribosomal subunit in the presence of crystallographically disordered codon and near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position
Overview
A structural and mechanistic explanation for the selection of tRNAs by the ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound at the decoding center and compare affinities of equivalent complexes in solution. In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove. As a result, the transition to a closed form of the 30S induced by cognate tRNA is unfavorable for near-cognate tRNA unless paromomycin induces part of the rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity.
About this Structure
1N36 is a Protein complex structure of sequences from Thermus thermophilus with as ligand. Full crystallographic information is available from OCA.
Reference
Selection of tRNA by the ribosome requires a transition from an open to a closed form., Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V, Cell. 2002 Nov 27;111(5):721-32. PMID:12464183
Page seeded by OCA on Thu Feb 21 14:01:43 2008
Categories: Protein complex | Thermus thermophilus | IV, F V.Murphy. | Ogle, J M. | Ramakrishnan, V. | Tarry, M J. | ZN | 30s ribosomal subunit | A site | Antibiotic | Anticodon | Codon | Decoding | G:u | Gu | Messenger rna | Mismatch | Mrna | Near-cognate | Paromomycin | Ribosome | Stem-loop | Transfer rna | Trna | Wobble
