1ndn
From Proteopedia
(New page: 200px<br /><applet load="1ndn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ndn, resolution 3.000Å" /> '''MOLECULAR STRUCTURE...) |
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| - | [[Image:1ndn.jpg|left|200px]]<br /><applet load="1ndn" size=" | + | [[Image:1ndn.jpg|left|200px]]<br /><applet load="1ndn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ndn, resolution 3.000Å" /> | caption="1ndn, resolution 3.000Å" /> | ||
'''MOLECULAR STRUCTURE OF NICKED DNA. MODEL T4'''<br /> | '''MOLECULAR STRUCTURE OF NICKED DNA. MODEL T4'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The molecular structure of a nicked dodecamer DNA double helix, made of a | + | The molecular structure of a nicked dodecamer DNA double helix, made of a ternary system containing d(CGCGAAAACGCG) + d(CGCGTT) + d(TTCGCG) oligonucleotides, has been determined by x-ray diffraction analysis at 3 A resolution. The molecule adopts a B-DNA conformation, not unlike those found in intact dodecamer DNA molecules crystallized in a somewhat different crystal lattice, despite a gap due to the absence of a phosphate group in the molecule. The helix has a distinct narrow minor groove near the center of the molecule at the AAAA region. This suggests that the internal stabilizing forces due to base stacking and hydrogen-bonding interactions are sufficient to overcome the loss of connectivity associated with the disruption of the covalent backbone of DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1NDN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1NDN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NDN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Aymani, J.]] | [[Category: Aymani, J.]] | ||
| - | [[Category: Boom, J | + | [[Category: Boom, J H.Van.]] |
[[Category: Coll, M.]] | [[Category: Coll, M.]] | ||
| - | [[Category: Marel, G | + | [[Category: Marel, G A.Van Der.]] |
[[Category: Rich, A.]] | [[Category: Rich, A.]] | ||
| - | [[Category: Wang, A | + | [[Category: Wang, A H.J.]] |
[[Category: b-dna]] | [[Category: b-dna]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: nicked]] | [[Category: nicked]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:03 2008'' |
Revision as of 12:05, 21 February 2008
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MOLECULAR STRUCTURE OF NICKED DNA. MODEL T4
Overview
The molecular structure of a nicked dodecamer DNA double helix, made of a ternary system containing d(CGCGAAAACGCG) + d(CGCGTT) + d(TTCGCG) oligonucleotides, has been determined by x-ray diffraction analysis at 3 A resolution. The molecule adopts a B-DNA conformation, not unlike those found in intact dodecamer DNA molecules crystallized in a somewhat different crystal lattice, despite a gap due to the absence of a phosphate group in the molecule. The helix has a distinct narrow minor groove near the center of the molecule at the AAAA region. This suggests that the internal stabilizing forces due to base stacking and hydrogen-bonding interactions are sufficient to overcome the loss of connectivity associated with the disruption of the covalent backbone of DNA.
About this Structure
1NDN is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Molecular structure of nicked DNA: a substrate for DNA repair enzymes., Aymami J, Coll M, van der Marel GA, van Boom JH, Wang AH, Rich A, Proc Natl Acad Sci U S A. 1990 Apr;87(7):2526-30. PMID:2320572
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