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1njg
From Proteopedia
(New page: 200px<br /><applet load="1njg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1njg, resolution 2.20Å" /> '''Nucleotide-free form...) |
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| - | [[Image:1njg.gif|left|200px]]<br /><applet load="1njg" size=" | + | [[Image:1njg.gif|left|200px]]<br /><applet load="1njg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1njg, resolution 2.20Å" /> | caption="1njg, resolution 2.20Å" /> | ||
'''Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit'''<br /> | '''Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. | + | Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process. |
==About this Structure== | ==About this Structure== | ||
| - | 1NJG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | + | 1NJG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJG OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Donnell, M | + | [[Category: Donnell, M O.]] |
[[Category: Kuriyan, J.]] | [[Category: Kuriyan, J.]] | ||
[[Category: Podobnik, M.]] | [[Category: Podobnik, M.]] | ||
| - | [[Category: Weitze, T | + | [[Category: Weitze, T F.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: sensor 2]] | [[Category: sensor 2]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:50 2008'' |
Revision as of 12:06, 21 February 2008
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Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit
Overview
Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process.
About this Structure
1NJG is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:12623013
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