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1ofx

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(New page: 200px<br /><applet load="1ofx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ofx, resolution 2.000&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1ofx.jpg|left|200px]]<br /><applet load="1ofx" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1ofx, resolution 2.000&Aring;" />
caption="1ofx, resolution 2.000&Aring;" />
'''CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION'''<br />
'''CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION'''<br />
==Overview==
==Overview==
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In DNA replication, Okazaki fragments are formed as double-stranded, intermediates during synthesis of the lagging strand. They are composed of, the growing DNA strand primed by RNA and the template strand. The DNA, oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide, r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and, its three-dimensional structure was determined by x-ray crystallography., The fragment adopts an overall A-type conformation with 11 residues per, turn. Although the base-pair geometry, particularly in the central TATA, part, is distorted, there is no evidence for a transition from the A- to, the B-type conformation at the junction between RNA.DNA hybrid and DNA, duplex. The RNA trimer may, therefore, lock the complete fragment in an, A-type conformation.
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In DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation.
==About this Structure==
==About this Structure==
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1OFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OFX OCA].
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1OFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFX OCA].
==Reference==
==Reference==
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[[Category: double helix]]
[[Category: double helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:51:56 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:22 2008''

Revision as of 12:17, 21 February 2008

Image:1ofx.jpg

1ofx, resolution 2.000Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION

Overview

In DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation.

About this Structure

1OFX is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of an Okazaki fragment at 2-A resolution., Egli M, Usman N, Zhang SG, Rich A, Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):534-8. PMID:1370582

Page seeded by OCA on Thu Feb 21 14:17:22 2008

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