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1ovv
From Proteopedia
(New page: 200px<br /><applet load="1ovv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ovv, resolution 2.90Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1ovv.jpg|left|200px]]<br /><applet load="1ovv" size=" | + | [[Image:1ovv.jpg|left|200px]]<br /><applet load="1ovv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ovv, resolution 2.90Å" /> | caption="1ovv, resolution 2.90Å" /> | ||
'''CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form II)'''<br /> | '''CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form II)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Diffraction data from two non-isomorphous crystals (forms 1 and 2) of an | + | Diffraction data from two non-isomorphous crystals (forms 1 and 2) of an artificial protein with a four-helix bundle motif, di-Co(II)-DF1-L13A, have been collected using synchrotron radiation. The phase of form 1 has been assigned using the group and minimal non-isomorphic supergroup relation between the space group of the previously determined di-Mn(II)-DF1-L13G structure and the space group of this form. This unconventional method of solving the phase problem has also been tested with form 2 using a reverse relation. The structure of the latter form has been solved using the group and maximal non-isomorphic subgroup relation with the space group of form 2 of the analogous dimanganese protein. This application has shown that this phasing method can be used for solving the protein structures of polymorphic crystals as an alternative to the molecular-replacement method. |
==About this Structure== | ==About this Structure== | ||
| - | 1OVV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CO, ACE and NH2 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1OVV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CO:'>CO</scene>, <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OVV OCA]. |
==Reference== | ==Reference== | ||
Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12876346 12876346] | Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12876346 12876346] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Costanzo, L | + | [[Category: Costanzo, L Di.]] |
[[Category: Geremia, S.]] | [[Category: Geremia, S.]] | ||
[[Category: ACE]] | [[Category: ACE]] | ||
| Line 20: | Line 20: | ||
[[Category: protein design]] | [[Category: protein design]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:22:08 2008'' |
Revision as of 12:22, 21 February 2008
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CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form II)
Overview
Diffraction data from two non-isomorphous crystals (forms 1 and 2) of an artificial protein with a four-helix bundle motif, di-Co(II)-DF1-L13A, have been collected using synchrotron radiation. The phase of form 1 has been assigned using the group and minimal non-isomorphic supergroup relation between the space group of the previously determined di-Mn(II)-DF1-L13G structure and the space group of this form. This unconventional method of solving the phase problem has also been tested with form 2 using a reverse relation. The structure of the latter form has been solved using the group and maximal non-isomorphic subgroup relation with the space group of form 2 of the analogous dimanganese protein. This application has shown that this phasing method can be used for solving the protein structures of polymorphic crystals as an alternative to the molecular-replacement method.
About this Structure
1OVV is a Protein complex structure of sequences from [1] with , and as ligands. Full crystallographic information is available from OCA.
Reference
Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:12876346
Page seeded by OCA on Thu Feb 21 14:22:08 2008
