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| - | [[Image:1wb3.gif|left|200px]]<br /> | + | #REDIRECT [[4acb]] This PDB entry is obsolete and replaced by 4acb |
| - | <applet load="1wb3" size="450" color="white" frame="true" align="right" spinBox="true"
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| - | caption="1wb3, resolution 3.20Å" />
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| - | '''CRYSTAL STRUCTURE OF TRANSLATION ELONGATION FACTOR SELB FROM METHANOCOCCUS MARIPALUDIS IN COMPLEX WITH THE GTP ANALOGUE GPPNHP'''<br />
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| - | ==Overview==
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| - | In all three kingdoms of life, SelB is a specialized translation, elongation factor responsible for the cotranslational incorporation of, selenocysteine into proteins by recoding of a UGA stop codon in the, presence of a downstream mRNA hairpin loop. Here, we present the X-ray, structures of SelB from the archaeon Methanococcus maripaludis in the, apo-, GDP- and GppNHp-bound form and use mutational analysis to, investigate the role of individual amino acids in its aminoacyl-binding, pocket. All three SelB structures reveal an EF-Tu:GTP-like domain, arrangement. Upon binding of the GTP analogue GppNHp, a conformational, change of the Switch 2 region in the GTPase domain leads to the exposure, of SelB residues involved in clamping the 5' phosphate of the tRNA. A, conserved extended loop in ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15616587 (full description)]]
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| - | ==About this Structure==
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| - | 1WB3 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Methanococcus_maripaludis Methanococcus maripaludis]] with MG, SO4, GNP and DXC as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WB3 OCA]].
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| - | ==Reference==
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| - | Selenocysteine tRNA-specific elongation factor SelB is a structural chimaera of elongation and initiation factors., Leibundgut M, Frick C, Thanbichler M, Bock A, Ban N, EMBO J. 2005 Jan 12;24(1):11-22. Epub 2004 Dec 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15616587 15616587]
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| - | [[Category: Methanococcus maripaludis]]
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| - | [[Category: Single protein]]
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| - | [[Category: Ban, N.]]
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| - | [[Category: Boeck, A.]]
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| - | [[Category: Frick, C.]]
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| - | [[Category: Leibundgut, M.]]
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| - | [[Category: Thanbichler, M.]]
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| - | [[Category: DXC]]
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| - | [[Category: GNP]]
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| - | [[Category: MG]]
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| - | [[Category: SO4]]
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| - | [[Category: protein synthesis]]
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| - | [[Category: ribosome]]
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| - | [[Category: selenium]]
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| - | [[Category: selenocysteine]]
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| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:58:47 2007''
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