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1pxw
From Proteopedia
(New page: 200px<br /><applet load="1pxw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pxw, resolution 1.94Å" /> '''Crystal structure of...) |
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| - | [[Image:1pxw.jpg|left|200px]]<br /><applet load="1pxw" size=" | + | [[Image:1pxw.jpg|left|200px]]<br /><applet load="1pxw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pxw, resolution 1.94Å" /> | caption="1pxw, resolution 1.94Å" /> | ||
'''Crystal structure of L7Ae sRNP core protein from Pyrococcus abyssii'''<br /> | '''Crystal structure of L7Ae sRNP core protein from Pyrococcus abyssii'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The L7Ae sRNP core protein from Pyrococcus abyssii was crystallized using | + | The L7Ae sRNP core protein from Pyrococcus abyssii was crystallized using the sitting-drop vapour-diffusion method. Crystals were obtained in the presence of MgCl(2), PEG 2000 MME and acetate buffer at pH 4.0. A native data set has been collected at 2.9 A resolution using a rotating-anode generator at room temperature. Crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 70.7, b = 112.9, c = 34.8 A. There are two monomers of MW 14 200 Da per asymmetric unit and the packing density V(M) is 2.45 A(3) Da(-1). A molecular-replacement analysis gave solutions for the rotation and translation functions. |
==About this Structure== | ==About this Structure== | ||
| - | 1PXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi]. Full crystallographic information is available from [http:// | + | 1PXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PXW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ribosome]] | [[Category: ribosome]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:43 2008'' |
Revision as of 12:33, 21 February 2008
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Crystal structure of L7Ae sRNP core protein from Pyrococcus abyssii
Overview
The L7Ae sRNP core protein from Pyrococcus abyssii was crystallized using the sitting-drop vapour-diffusion method. Crystals were obtained in the presence of MgCl(2), PEG 2000 MME and acetate buffer at pH 4.0. A native data set has been collected at 2.9 A resolution using a rotating-anode generator at room temperature. Crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 70.7, b = 112.9, c = 34.8 A. There are two monomers of MW 14 200 Da per asymmetric unit and the packing density V(M) is 2.45 A(3) Da(-1). A molecular-replacement analysis gave solutions for the rotation and translation functions.
About this Structure
1PXW is a Single protein structure of sequence from Pyrococcus abyssi. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary X-ray diffraction data of L7Ae sRNP core protein from Pyrococcus abyssii., Charron C, Manival X, Charpentier B, Branlant C, Aubry A, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):122-4. Epub 2003, Dec 18. PMID:14684904
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